CAJ | 학술논문

目的：探讨过氧化物酶体增殖因子活化受体gamma( PPAR-γ)激动剂对腭中缝扩张( MSE)后骨重建的影响。方法应用雄性野生型C57BL/6小鼠共60只,分为假手术+正常饮食组；MSE+正常饮食组；MSE+匹格列酮饮食组。术后14d或28d处死,采用HE染色,实时荧光定量PCR方法研究匹格列酮对腭中缝扩张后骨重建的影响。结果 PPAR-γ激动剂匹格列酮减少腭中缝扩张手术后小鼠腭中缝成骨,并显著下调成骨细胞调控分子(Runx2和Dxl5)以及成骨细胞标志分子(COLIα1)的基因表达。结论在快速上颌扩大术后的腭中缝牵张成骨过程中,PPAR-γ激动剂匹格列酮抑制新骨形成；其机制可能是通过抑制成骨细胞的分化。
목적：탐토과양화물매체증식인자활화수체gamma( PPAR-γ)격동제대악중봉확장( MSE)후골중건적영향。방법응용웅성야생형C57BL/6소서공60지,분위가수술+정상음식조；MSE+정상음식조；MSE+필격렬동음식조。술후14d혹28d처사,채용HE염색,실시형광정량PCR방법연구필격렬동대악중봉확장후골중건적영향。결과 PPAR-γ격동제필격렬동감소악중봉확장수술후소서악중봉성골,병현저하조성골세포조공분자(Runx2화Dxl5)이급성골세포표지분자(COLIα1)적기인표체。결론재쾌속상합확대술후적악중봉견장성골과정중,PPAR-γ격동제필격렬동억제신골형성；기궤제가능시통과억제성골세포적분화。
Objective To investigate the impact of agonist of peroxisome proliferator-activated receptor-gamma ( PPAR-g) on bone remodeling after midpalatal suture expansion ( MSE ) . Methods Sixty wild type male mice were divided into three groups, sham operation with normal diet, MSE with normal diet, and MSE with pioglitazone diet. The mice were killed 14 or 28 days after sham operation or MSE. HE staining and real time quantitative PCR were used to study the effects of pioglitazone on bone remodeling after MSE. Results Pioglitazone reduced the amount of cells and new bone formation in the midpalatal suture and down-regulated the expression of genes that control the differentiation of osteoblasts (including Runx2 and Dxl5) and of osteoblast marker genes (including COLIα1). Conclusion In the process of bone remodeling after MSE, PPAR-g agonist pioglitazone decreases new bone formation, likely through inhibition of the differentiation of osteoblasts.