中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2014年
7期
77-80
,共4页
黄爱文%赵佳丽%刘志宏%曾棋平%宋洪涛
黃愛文%趙佳麗%劉誌宏%曾棋平%宋洪濤
황애문%조가려%류지굉%증기평%송홍도
巯基化透明质酸%胰岛素%纳米粒
巰基化透明質痠%胰島素%納米粒
구기화투명질산%이도소%납미립
thiolated hyaluronic acid%insulin%nanoparticles
目的:制备胰岛素巯基化透明质酸纳米粒(insulin thiolated hyaluronic acid nanoparticles,Ins-HA-Cys-NPs),考察纳米粒的理化性质。方法以合成的具有生物黏附性质的巯基化透明质酸作为载体,采用超声乳化法制备纳米粒,考察其外观、粒径、Zeta电位、包封率、载药量,并进行其冻干制剂的冻干保护剂筛选。结果制备的Ins-HA-Cys-NPs粒径均一,外观圆整;平均粒径为(178.5±0.8)nm,PDI为(0.214±0.013),Zeta电位为-(38.47±0.46)mV,超滤离心法测定载药纳米粒的包封率为(48.85±0.66)%,载药量为(4.79±0.13)%;选择10%的甘露醇为冻干保护剂,复溶后得到具有蓝色乳光的粒径均一的纳米粒混悬液。结论巯基化透明质酸纳米粒是蛋白多肽类药物口服给药的潜在载体,为下一步研究胰岛素纳米粒在大鼠体内药效、药动学研究提供前提和基础。
目的:製備胰島素巰基化透明質痠納米粒(insulin thiolated hyaluronic acid nanoparticles,Ins-HA-Cys-NPs),攷察納米粒的理化性質。方法以閤成的具有生物黏附性質的巰基化透明質痠作為載體,採用超聲乳化法製備納米粒,攷察其外觀、粒徑、Zeta電位、包封率、載藥量,併進行其凍榦製劑的凍榦保護劑篩選。結果製備的Ins-HA-Cys-NPs粒徑均一,外觀圓整;平均粒徑為(178.5±0.8)nm,PDI為(0.214±0.013),Zeta電位為-(38.47±0.46)mV,超濾離心法測定載藥納米粒的包封率為(48.85±0.66)%,載藥量為(4.79±0.13)%;選擇10%的甘露醇為凍榦保護劑,複溶後得到具有藍色乳光的粒徑均一的納米粒混懸液。結論巰基化透明質痠納米粒是蛋白多肽類藥物口服給藥的潛在載體,為下一步研究胰島素納米粒在大鼠體內藥效、藥動學研究提供前提和基礎。
목적:제비이도소구기화투명질산납미립(insulin thiolated hyaluronic acid nanoparticles,Ins-HA-Cys-NPs),고찰납미립적이화성질。방법이합성적구유생물점부성질적구기화투명질산작위재체,채용초성유화법제비납미립,고찰기외관、립경、Zeta전위、포봉솔、재약량,병진행기동간제제적동간보호제사선。결과제비적Ins-HA-Cys-NPs립경균일,외관원정;평균립경위(178.5±0.8)nm,PDI위(0.214±0.013),Zeta전위위-(38.47±0.46)mV,초려리심법측정재약납미립적포봉솔위(48.85±0.66)%,재약량위(4.79±0.13)%;선택10%적감로순위동간보호제,복용후득도구유람색유광적립경균일적납미립혼현액。결론구기화투명질산납미립시단백다태류약물구복급약적잠재재체,위하일보연구이도소납미립재대서체내약효、약동학연구제공전제화기출。
Objective To prepare insulin thiolated hyaluronic acid nanoparticles (Ins-HA-Cys-NPs)and study its physicochemical properties. Methods The Ins-HA-Cys-NPs was prepared by ultrasonic emulsifying method,and the properties of nanoparticles including morphology,mean diameter,Zeta potential,entrapment efficiency and drug loading efficiency were studied,as well as the cryoprotectant selection.Results The prepared nanoparticles was round in appearance and the mean diameter was(178.5 ±0.8)nm,the polydispersity index was (0.214 ±0.013)and the Zeta potential was -(38.47 ±0.46 )mV,while the entrapment efficiency was (48.85 ±0.66 )%,drug loading efficiency was (4.79 ±0.13 )%;10%mannitol as cryoprotectant provided uniform and well dispersed suspension of nanoparticles with blue opalescence after redispersion.Conclusion The thiolated hyaluronic acid nanoparticles may be used as the carrier for oral drug delivery system of insulin,and it provides a basis for studies on rats in vivo.