CAJ | 학술논문

目的：探讨纳米二氧化硅是否可作为生物大分子药物口服载体。方法研究钙离子的量对二氧化硅纳米颗粒形态的影响、不同钙离子存在下二氧化硅纳米颗粒对蛋白或多肽的包裹率、蛋白或多肽从二氧化硅纳米颗粒中释放情况、二氧化硅包裹蛋白酶解情况、以及二氧化硅纳米颗粒包裹125 I标记多肽在小鼠体内释放情况。结果实验表明合成的二氧化硅纳米颗粒包裹多肽在小白鼠体内可以顺利通过胃酸到达小肠，有作为生物活性大分子口服药物载体的潜力。结论蛋白或多肽比较容易在碱性条件下释放，在pH 2的酸性条件下释放极少（胃酸的pH值），制备的二氧化硅纳米颗粒有作为口服药物载体的潜力。
목적：탐토납미이양화규시부가작위생물대분자약물구복재체。방법연구개리자적량대이양화규납미과립형태적영향、불동개리자존재하이양화규납미과립대단백혹다태적포과솔、단백혹다태종이양화규납미과립중석방정황、이양화규포과단백매해정황、이급이양화규납미과립포과125 I표기다태재소서체내석방정황。결과실험표명합성적이양화규납미과립포과다태재소백서체내가이순리통과위산도체소장，유작위생물활성대분자구복약물재체적잠력。결론단백혹다태비교용역재감성조건하석방，재pH 2적산성조건하석방겁소（위산적pH치），제비적이양화규납미과립유작위구복약물재체적잠력。
Objective To study whether the silica nanoparticles can be used as a biomacromolecular carrier of oral drugs.Methods Studyed the influence of the amount of calcium ions on nano silica nanoparticles morphology,the encapsulation rate of silica nanoparticles on proteins or peptides in the presence of different calcium ions,the situation of proteins or peptides released from silica nanoparticles,enzymolysis of protein packaged by silica, silica nanoparticles labeled peptides with 125 I released in mice.Results Experiments showed that the synthetic silica nanoparticles packaged with peptides could smoothly reach the small intestine through the gastric acid in mice, it had potential to be biomacromolecular carrier of oral drug. Conclusion Proteins or peptides is easy to release in alkaline condition,but release few in acidic condition of pH2 (PH of gastric acid).The silica nanoparticles prepared have the potential to be oral drug carrier.