中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2014年
7期
61-65
,共5页
帕瑞昔布%COX-2%Caspase-3%海马%炎症
帕瑞昔佈%COX-2%Caspase-3%海馬%炎癥
파서석포%COX-2%Caspase-3%해마%염증
parecoxib%COX-2%Caspase-3%hippocampus%inflammation
目的:研究帕瑞昔布对肝部分切除后大鼠海马炎症反应的影响及可能机制。方法选取72只SPF级雄性健康SD大鼠,随机分为对照组(C组)、麻醉组(A组)、手术组(S组)、帕瑞昔布组(P组)4组,每组18只。采用肝部分切除术建立手术创伤致海马炎症动物模型。麻醉后1、3、7 d处死大鼠,检测海马组织炎症介质的表达。采用实时定量PCR检测环氧合酶-2(COX-2)mRNA的表达量,Western blot检测COX-2、半胱氨酸蛋白酶-3(Caspase-3)的蛋白表达量,酶联免疫吸附法(ELISA)检测前列腺素E2(PGE2)的含量。结果与C组相比,A组大鼠海马组织中COX-2表达(蛋白、mRNA),PGE2含量,Caspase-3蛋白表达均差异均无统计学意义;S组、P组大鼠术后第1、3天COX-2,PGE2,Caspase-3表达均明显上调(P<0.05,P<0.01),尤以术后第1天明显(P<0.01),术后第7天上述各炎症指标表达恢复正常。与S组相比,P组术后第1、3天COX-2,PGE2,Caspase-3表达明显下调(P<0.05,P<0.01)。结论肝部分切除术可导致海马炎症反应,帕瑞昔布钠可显著抑制此种炎症反应,其机制可能与下调海马COX-2 mRNA和蛋白表达,降低PGE2含量和Caspase-3蛋白表达有关。
目的:研究帕瑞昔佈對肝部分切除後大鼠海馬炎癥反應的影響及可能機製。方法選取72隻SPF級雄性健康SD大鼠,隨機分為對照組(C組)、痳醉組(A組)、手術組(S組)、帕瑞昔佈組(P組)4組,每組18隻。採用肝部分切除術建立手術創傷緻海馬炎癥動物模型。痳醉後1、3、7 d處死大鼠,檢測海馬組織炎癥介質的錶達。採用實時定量PCR檢測環氧閤酶-2(COX-2)mRNA的錶達量,Western blot檢測COX-2、半胱氨痠蛋白酶-3(Caspase-3)的蛋白錶達量,酶聯免疫吸附法(ELISA)檢測前列腺素E2(PGE2)的含量。結果與C組相比,A組大鼠海馬組織中COX-2錶達(蛋白、mRNA),PGE2含量,Caspase-3蛋白錶達均差異均無統計學意義;S組、P組大鼠術後第1、3天COX-2,PGE2,Caspase-3錶達均明顯上調(P<0.05,P<0.01),尤以術後第1天明顯(P<0.01),術後第7天上述各炎癥指標錶達恢複正常。與S組相比,P組術後第1、3天COX-2,PGE2,Caspase-3錶達明顯下調(P<0.05,P<0.01)。結論肝部分切除術可導緻海馬炎癥反應,帕瑞昔佈鈉可顯著抑製此種炎癥反應,其機製可能與下調海馬COX-2 mRNA和蛋白錶達,降低PGE2含量和Caspase-3蛋白錶達有關。
목적:연구파서석포대간부분절제후대서해마염증반응적영향급가능궤제。방법선취72지SPF급웅성건강SD대서,수궤분위대조조(C조)、마취조(A조)、수술조(S조)、파서석포조(P조)4조,매조18지。채용간부분절제술건립수술창상치해마염증동물모형。마취후1、3、7 d처사대서,검측해마조직염증개질적표체。채용실시정량PCR검측배양합매-2(COX-2)mRNA적표체량,Western blot검측COX-2、반광안산단백매-3(Caspase-3)적단백표체량,매련면역흡부법(ELISA)검측전렬선소E2(PGE2)적함량。결과여C조상비,A조대서해마조직중COX-2표체(단백、mRNA),PGE2함량,Caspase-3단백표체균차이균무통계학의의;S조、P조대서술후제1、3천COX-2,PGE2,Caspase-3표체균명현상조(P<0.05,P<0.01),우이술후제1천명현(P<0.01),술후제7천상술각염증지표표체회복정상。여S조상비,P조술후제1、3천COX-2,PGE2,Caspase-3표체명현하조(P<0.05,P<0.01)。결론간부분절제술가도치해마염증반응,파서석포납가현저억제차충염증반응,기궤제가능여하조해마COX-2 mRNA화단백표체,강저PGE2함량화Caspase-3단백표체유관。
Objective To investigate the effect of parecoxib on hippocampal inflammation following partial hepatectomy in rats.Methods 72 SPF male healthy SD rats were selected and randomly divided into four groups:control group(group C),anesthesia group(group A),operation group(group S),parecoxib group (group P ), each had 18 rats.Using partial hepatectomy operation trauma to establish animal model of hippocampal inflammation.Rats were sacrificed 1,3,7 days after anesthesia,the expression of inflammatory mediators in hippocampal tissue were detected.The expression of cyclooxygenase -2(COX-2)mRNA were detected by real-time quantitative PCR,and COX-2 and cysteine proteinase -3(Caspase-3)protein expression were detected by Western blot,the prostaglandin E2(PGE2)content were detected by enzyme linked immunosorbent assay(ELISA).Results Compared with control group,COX-2 expression(protein and mRNA),PGE2 production and Caspase-3 protein expression in group A had no significant difference.In group S and P,COX-2 expression,PGE2 and Caspase-3 protein expression increased significantly on 1st day postoperatively to 3rd day (P<0.05 or P<0.01),and they were obvious especially in the postoperative 1st day(P<0.01).The above inflammation index expression returned to normal in 7th days after operation.Compared with group S,COX-2 expression,PGE2 and Caspase-3 protein expression increased significantly on 1st day postoperatively to 3rd day(P <0.05 or P <0.01 ).Conclusion Partial hepatectomy could induce hippocampal inflammatory response in the rats.Parecoxib could suppress the hippocampal inflammation via the down-regulation expression of COX-2 mRNA and protein,PGE2 and Caspase-3 protein in rats.