CAJ | 학술논문

目的：研究干扰素-γ（IFN-γ）对肾癌细胞增殖干预的机制。方法应用浓度1000、2000、3000 U/mL的IFN-γ处理肾癌786-0细胞株，在处理24、48、72 h后，采用CCK-8法测定细胞增殖抑制率，采用流式细胞仪分析细胞周期，采用RT-PCR法检测肝细胞粘附分子（hepaCAM）mRNA的表达，采用Western bolt方法检测MAD1蛋白表达情况。结果不同浓度的IFN-γ对肾癌细胞的增殖均有抑制作用，相同浓度不同时间抑制率72h＞48 h＞24 h，差异有统计学意义（P＜0.05）；相同作用时间，IFN-γ浓度越大，抑制率越大，差异有统计学意义（P＜0.05）；细胞周期分布结果显示，实验组肾癌细胞在处理48 h后出现G0/G1期增殖异常；与对照组（39.89）比较，实验组增殖指数（25.65）明显下降，差异有统计学意义（P＜0.05）；结果显示，实验组肾癌细胞在处理48 h后，与对照组比较，hepaCAM、MAD1蛋白表达量明显升高，差异有统计学意义（P＜0.05）。结论干扰素-γ可以通过促进hepaCAM基因表达，使MAD1蛋白表达升高，阻碍肾癌细胞增殖过程，对临床具有指导意义。
목적：연구간우소-γ（IFN-γ）대신암세포증식간예적궤제。방법응용농도1000、2000、3000 U/mL적IFN-γ처리신암786-0세포주，재처리24、48、72 h후，채용CCK-8법측정세포증식억제솔，채용류식세포의분석세포주기，채용RT-PCR법검측간세포점부분자（hepaCAM）mRNA적표체，채용Western bolt방법검측MAD1단백표체정황。결과불동농도적IFN-γ대신암세포적증식균유억제작용，상동농도불동시간억제솔72h＞48 h＞24 h，차이유통계학의의（P＜0.05）；상동작용시간，IFN-γ농도월대，억제솔월대，차이유통계학의의（P＜0.05）；세포주기분포결과현시，실험조신암세포재처리48 h후출현G0/G1기증식이상；여대조조（39.89）비교，실험조증식지수（25.65）명현하강，차이유통계학의의（P＜0.05）；결과현시，실험조신암세포재처리48 h후，여대조조비교，hepaCAM、MAD1단백표체량명현승고，차이유통계학의의（P＜0.05）。결론간우소-γ가이통과촉진hepaCAM기인표체，사MAD1단백표체승고，조애신암세포증식과정，대림상구유지도의의。
Objective To study the mechanism of interferon-γ(IFN-γ)on the intervention of renal carcinoma cell proliferation.Methods Using concentration of 1 000,2 000,3 000 U/mL IFN-γtreatment of renal cell carcinoma 786-0 cell line,in 24 hours,48 hours,72 hours after treatment,the inhibition rate of cell proliferation was determined with CCK-8 method,using flow cytometric analysis of cell cycle,using RT-PCR for detection of hepaCAM mRNA,and using the Western boltting method for detection of MAD1 protein expression.Results Different concentrations of IFN-γhad the inhibitory effects on renal cell carcinoma cell proliferation,the concentration of the inhibitory rate of 72 hoursand 48 hours more than 24 hours,the difference was statistically significant (P<0.05);at the same time,a higher IFN-γconcentration,the inhibition rate was greater,the difference was statistically significant (P<0.05 );the cell cycle distribution results showed,the experimental group of renal carcinoma cells proliferation in the treatment of abnormal G0/G1 phase after 48 hours;and the control group (39.89 )compared with the experimental group,the proliferation index (25.65 )decreased significantly,the difference was statistically significant (P<0.05 );results showed that,the experimental group in renal cell carcinoma cells after 48 h of treatment,compared with control group,hepaCNM mRNA,MAD1 protein expression increased obviously,the difference was statistically significant (P<0.05 ).Conclusion IFN-γcould increase the expression of MAD1 by promoting hepaCAM expression,inhibits renal carcinoma cell proliferation.