CAJ | 학술논문

目的：研究分析大蒜素前药对食管癌细胞Eca9706的增殖及对凋亡基因表达的影响。方法不同浓度的大蒜素前药分为A1组（10μg/mL）、A2组（20μg/mL）、A3组（40μg/mL）、A4组（生理盐水，0μg/mL）共4组，分别作用于食管癌细胞Eca9706，在培养1、2、3 d后，用MTT比色法（噻唑蓝）检测细胞增殖情况，实时荧光定量（RT-PCR法）检测4组细胞p53基因在mRNA水平表达情况。结果大蒜素前药对食管癌细胞 Eca9706的增值抑制的最佳浓度为20μg/mL，最佳抑制时间为2 d，其对食管癌细胞Eca9706基因水平的抑制呈剂量依赖性。结论大蒜素前药可以有效抑制食管癌细胞Eca9706的增值，通过对凋亡相关基因的调控可以控制食管癌细胞增殖，从而达到消除肿瘤细胞的目的。
목적：연구분석대산소전약대식관암세포Eca9706적증식급대조망기인표체적영향。방법불동농도적대산소전약분위A1조（10μg/mL）、A2조（20μg/mL）、A3조（40μg/mL）、A4조（생리염수，0μg/mL）공4조，분별작용우식관암세포Eca9706，재배양1、2、3 d후，용MTT비색법（새서람）검측세포증식정황，실시형광정량（RT-PCR법）검측4조세포p53기인재mRNA수평표체정황。결과대산소전약대식관암세포 Eca9706적증치억제적최가농도위20μg/mL，최가억제시간위2 d，기대식관암세포Eca9706기인수평적억제정제량의뢰성。결론대산소전약가이유효억제식관암세포Eca9706적증치，통과대조망상관기인적조공가이공제식관암세포증식，종이체도소제종류세포적목적。
Objective To explore and analyze the effects of allicin prodrug on proliferation of esophageal cancer cell line Eca9706 and the expression of apoptosis gene.Methods Different concentrations of allicin prodrugs were divided into a total of four groups:A1 group(10μg/mL),A2 group(20 μg/mL),A3 group (40 μg/mL),A4 group (normal saline,0 μg/mL),and respectively applicated in esophageal carcinoma cell line Eca9706.After culturing for 1 days,2 days,3 days,cell proliferation was detected by MTT and expression of p53 at the mRNA level was detected by RT-PCR.Results The optimum concentration of proliferation inhibition of allicin prodrug on esophageal cancer cell line Eca9706 was 20μg/mL and the best inhibition time was 2 days;the gene level of esophageal cancer cell line Eca9706 was inhibited with a dose-dependent.Conclusion Allicin prodrugs could effectively inhibit the proliferation of esophageal cancer cell line Eca9706,and control the proliferation of esophageal cancer cells by regulating the expression of apoptosis associated genes,so as to eliminate tumor cells.