CAJ | 학술논문

目的：探讨三七总皂苷（totalsaponinsofpanaxnotoginseseng，PNS）对局灶性脑梗死大鼠海马CA1区神经元保护及促进神经重塑的机制。方法将动物随机分为Y27632组、PNS加Y27632组、假手术组和手术组，对除了假手术组外的所有动物，建立大鼠大脑中动脉阻塞（MCAO）模型，造模成功且未分组的动物根据Longa评分和体重进行随机分组，分别为模型组、PNS组、尼莫地平组。观察造模7d后大鼠一般活动状态、体重指数和海马CA1区神经元形态学及NogoA、NgR1和RhoA蛋白表达变化。结果与假手术组比较，模型组NogoA、NgR1和RhoA蛋白表达增多（P＜0．01）；与模型组比较，PNS组大鼠NogoA、NgR1和RhoA蛋白表达均下降（P＜0．05），PNS加Y27632组NgR1和RhoA蛋白表达降低（P＜0．05），Y27632组RhoA蛋白表达下降（P＜0．01）。结论PNS具有与Y27632相似的作用，对脑梗死后NogoA/NgR1/Rho通路的上下游均有调节作用，可促进脑梗死损伤后海马神经功能重塑。
목적：탐토삼칠총조감（totalsaponinsofpanaxnotoginseseng，PNS）대국조성뇌경사대서해마CA1구신경원보호급촉진신경중소적궤제。방법장동물수궤분위Y27632조、PNS가Y27632조、가수술조화수술조，대제료가수술조외적소유동물，건립대서대뇌중동맥조새（MCAO）모형，조모성공차미분조적동물근거Longa평분화체중진행수궤분조，분별위모형조、PNS조、니막지평조。관찰조모7d후대서일반활동상태、체중지수화해마CA1구신경원형태학급NogoA、NgR1화RhoA단백표체변화。결과여가수술조비교，모형조NogoA、NgR1화RhoA단백표체증다（P＜0．01）；여모형조비교，PNS조대서NogoA、NgR1화RhoA단백표체균하강（P＜0．05），PNS가Y27632조NgR1화RhoA단백표체강저（P＜0．05），Y27632조RhoA단백표체하강（P＜0．01）。결론PNS구유여Y27632상사적작용，대뇌경사후NogoA/NgR1/Rho통로적상하유균유조절작용，가촉진뇌경사손상후해마신경공능중소。
O bjective To study the effect o f to ta lsaponins o f panax no tognseng(PNS) injection on the rem o ldability and pro tec-tive m echanism in hippocam pus CA1 in ra ts w ith m iddle cerebra l artery occlusion (MCAO). M e thods The Y27632 g roup,PNS and Y27632 g roup and the contro lg roup w ere taken out random ly from a l SPF S prague D aw ley ra ts. A l o f the ra ts w ere used to estab-lish MCAO m ode l by im proving Longa M e thod except to the contro l g roup. Acco rding to Longa sco res and w e ights the successful m ode led and un g roup ra ts w ere random ly divided into m ode lg roup,PNS g roup and nim odipine(NP) g roup.A l o f ra ts w ere execu-ted 7 days after opera tion. The genera l sta te, w e ight and neuro log ica l function sco re o f the ra ts w ere assessed da ily.The pa tho log i-ca l and m o rpho log ica l and the expression o fNogo A, NgR1 and RhoA in hippocam pus CA1 w ere de tected.Results The expres-sion o fNogo A, NgR1 and RhoA in m ode l g roup w ere m o re than the contro l g roup P < 0.01). The expression o f Nogo A, NgR1 and RhoA in PNS g roup and the expression o fNgR1 and RhoA in PNS and Y27632 gourp w ere less than the modelgroup( P < 0.05 or( P< 0.01). The expression o fRhoA in Y27632 g roup w ere low er than the m ode lg roup( P < 0.01). Conclusion The PNS injection has the sam e effects as Y27632 and regula tes bo th the upstream and dow nstream o fNogo A /NgR1/RhoA signa ling pa thw ay, so it can enhance the recovery o f hippocam pus CA1 neuro log ica l function o f the MCAO ra ts.