中西医结合心脑血管病杂志
中西醫結閤心腦血管病雜誌
중서의결합심뇌혈관병잡지
CHINESE JOURNAL OF INTEGRATIVE MEDICINE ON CARDIO-/CEREBROVASCULAR DISEASE
2014年
11期
1358-1360
,共3页
心肌缺血再灌注%炎症%缩醛基毛冬青提取化合物 R4
心肌缺血再灌註%炎癥%縮醛基毛鼕青提取化閤物 R4
심기결혈재관주%염증%축철기모동청제취화합물 R4
myocardialischemia reperfusion%inflammatory%MDQR4
目的:观察缩醛基毛冬青提取化合物 R4对在体大鼠急性心肌缺血/再灌注损伤( I/R)的影响,并探讨其保护作用机制。方法建立大鼠心肌缺血/再灌注模型,采用试剂盒测定再灌注40 m in后血清肌酸激酶(CK)、肿瘤坏死因子(TNF α)、IL 1Β的水平,观察心肌梗死范围,采用凝胶电泳法测定核转录因子κB(NF κB)的活性,采用试剂盒测定测定心肌组织超氧化物( SOD)活性及丙二醛(MDA)含量。结果缺血20 m in再灌注40 m in后模型组及用药组的血清 TNF α、IL 1β、CK水平明显高于假手术组,且缩醛基毛冬青提取化合物 R 4组及维拉帕米对照组明显低于模型组。再灌注40m in后模型组及用药组的梗死范围明显增加, NF κB表达明显增高,且缩醛基毛冬青提取化合物 R4组及维拉帕米对照组明显低于模型组。再灌注40 m in后模型组及用药组的SOD明显降低,MDA明显增高,缩醛基毛冬青提取化合物 R4组及维拉帕米对照组 MDA明显低于模型组,SOD明显高于模型组,有统计学意义。结论缩醛基毛冬青提取化合物 R4对缺血再灌注心肌的保护作用与其显著缩小心肌梗死面积,降低肌酸磷酸激酶,降低 MDA同时提高 SOD ,减少心肌细胞内 TNF α、IL 1β、NF κB蛋白的表达有关。
目的:觀察縮醛基毛鼕青提取化閤物 R4對在體大鼠急性心肌缺血/再灌註損傷( I/R)的影響,併探討其保護作用機製。方法建立大鼠心肌缺血/再灌註模型,採用試劑盒測定再灌註40 m in後血清肌痠激酶(CK)、腫瘤壞死因子(TNF α)、IL 1Β的水平,觀察心肌梗死範圍,採用凝膠電泳法測定覈轉錄因子κB(NF κB)的活性,採用試劑盒測定測定心肌組織超氧化物( SOD)活性及丙二醛(MDA)含量。結果缺血20 m in再灌註40 m in後模型組及用藥組的血清 TNF α、IL 1β、CK水平明顯高于假手術組,且縮醛基毛鼕青提取化閤物 R 4組及維拉帕米對照組明顯低于模型組。再灌註40m in後模型組及用藥組的梗死範圍明顯增加, NF κB錶達明顯增高,且縮醛基毛鼕青提取化閤物 R4組及維拉帕米對照組明顯低于模型組。再灌註40 m in後模型組及用藥組的SOD明顯降低,MDA明顯增高,縮醛基毛鼕青提取化閤物 R4組及維拉帕米對照組 MDA明顯低于模型組,SOD明顯高于模型組,有統計學意義。結論縮醛基毛鼕青提取化閤物 R4對缺血再灌註心肌的保護作用與其顯著縮小心肌梗死麵積,降低肌痠燐痠激酶,降低 MDA同時提高 SOD ,減少心肌細胞內 TNF α、IL 1β、NF κB蛋白的錶達有關。
목적:관찰축철기모동청제취화합물 R4대재체대서급성심기결혈/재관주손상( I/R)적영향,병탐토기보호작용궤제。방법건립대서심기결혈/재관주모형,채용시제합측정재관주40 m in후혈청기산격매(CK)、종류배사인자(TNF α)、IL 1Β적수평,관찰심기경사범위,채용응효전영법측정핵전록인자κB(NF κB)적활성,채용시제합측정측정심기조직초양화물( SOD)활성급병이철(MDA)함량。결과결혈20 m in재관주40 m in후모형조급용약조적혈청 TNF α、IL 1β、CK수평명현고우가수술조,차축철기모동청제취화합물 R 4조급유랍파미대조조명현저우모형조。재관주40m in후모형조급용약조적경사범위명현증가, NF κB표체명현증고,차축철기모동청제취화합물 R4조급유랍파미대조조명현저우모형조。재관주40 m in후모형조급용약조적SOD명현강저,MDA명현증고,축철기모동청제취화합물 R4조급유랍파미대조조 MDA명현저우모형조,SOD명현고우모형조,유통계학의의。결론축철기모동청제취화합물 R4대결혈재관주심기적보호작용여기현저축소심기경사면적,강저기산린산격매,강저 MDA동시제고 SOD ,감소심기세포내 TNF α、IL 1β、NF κB단백적표체유관。
O bjective To study the effect o fMDQR4 on acute m yocardia l ischem ia reperfusion( I/R) in rats. Methods M anufac-ture the m ode l o fm yocardia l I/R in rats. U sing kit to observe the leve ls o f serum crea tine kinase(CK) ,tum o r necrosis facto r a lpha (TNF α) and interleukin 1β( IL 1β). and the infarct size o f each group w ere m easured by TTC sta ining. And the leve l o f NF κB w as a lso de tected.And contento fm a londia ldehyde(MDA) and superoxide dism utase(SOD) in m yocardia l tissue w ere observed. Re-sults Fo low ing ischem ia 20m in and reperfusion 40m in,serum concentra tion o f TNF α ,IL 1β and CK in I/R group and m edicine pre trea tm ent group w as significantly increased com pared w ith tha t in sham group,and serum concentra tion o fTNF α ,IL 1β and CK in every m edicine pre trea tm ent group w as significantly low er than tha t in I/R group. Fo low ing ischem ia 20 m in and reperfusion 40 m in,infarct size and the expression o fNF κB in I/R group and m edicine pre trea tm ent group w as significantly increased. And infarct size and the expression o f NF κB in every m edicine pre trea tm ent group w as significantly low er than tha t in I/R group. Fo low ing reperfusion 40m in ,MDA contentw as significantly increased and SOD contentw as significantly reduced com pared w ith tha t in sham group,and MDA content in every m edicine pre trea tm ent group w as significantly low er than tha t in I/R group ,and SOD content in ev-ery m edicine pre trea tm ent group w as significantly higher than tha t in I/R group. Conclusion MDQR4 could reduce the infarct size and inhibitNF κB activa tion and dow n regula te the expression o f TNF α ,IL 1β ,CK ,MDA and increase the content o f SOD ,w hich m ay be one o f the m echanism s o f its cardiopro tection.