CAJ | 학술논문

目的：探讨心络绌急大鼠心肌细胞凋亡及 Bax、Bcl 2蛋白表达情况。方法采用垂体后叶素大剂量静脉注射建立心络绌急大鼠模型，运用 TUNEL法检测心肌细胞凋亡指数，免疫组化法检测心肌 Bax、Bcl 2、p53蛋白表达。结果与正常组相比，模型组大鼠心肌细胞凋亡指数显著升高（ P ＜0．01），Bax、p53蛋白表达显著增加（ P ＜0．05或 P ＜0．01），Bcl 2蛋白表达无明显变化，Bax/Bcl 2显著升高（ P ＜0．01）。结论细胞凋亡是心络绌急心肌损伤的重要原因，p53、Bax、Bcl 2表达的变化参与了心络绌急心肌细胞凋亡的调控。
목적：탐토심락출급대서심기세포조망급 Bax、Bcl 2단백표체정황。방법채용수체후협소대제량정맥주사건립심락출급대서모형，운용 TUNEL법검측심기세포조망지수，면역조화법검측심기 Bax、Bcl 2、p53단백표체。결과여정상조상비，모형조대서심기세포조망지수현저승고（ P ＜0．01），Bax、p53단백표체현저증가（ P ＜0．05혹 P ＜0．01），Bcl 2단백표체무명현변화，Bax/Bcl 2현저승고（ P ＜0．01）。결론세포조망시심락출급심기손상적중요원인，p53、Bax、Bcl 2표체적변화삼여료심락출급심기세포조망적조공。
O bjective To explo re expressions o f Bax, Bcl 2 and p53 o fm yocardium in ra ts w ith contraction o f heart colaterals. M e thods The anim a lm ode lw ith contraction o f heart co la tera ls w as established in ra ts by sing le high dose intravenous injection o f pituitrin. The apopto tic index, the expression o fBax,Bcl 2 and p53 o fm yocardium w ere respective ly de term ined by term ina ldeoxy-nucleo tide transferase m edia ted dUTP nick end labe ling ( TUNEL) assays and imm unohistochem ica l sta ining. Results C om-pared w ith no rm a lg roup, the apoptosis index,the expression o fBax, p53 and the ra tio o fBax/Bcl 2 in m ode lg roup w ere significantly increased ( P < 0.05 o r P < 0.01). Conclusion The m yocardia l apoptosis w as invo lved in the pa tho log ica l process o fm yocardia l ischem ia injury w ith contraction o f heart colaterals. Expressions o f Bax, Bcl 2 and p53 o fm yocardium w ere invo lved in regula ting apoptosis o fm yocardium in ra ts w ith contraction o f heart colaterals.