CAJ | 학술논문

目的：优选丹参-人参活性组分抗乳腺癌的最佳配伍，初步研究其作用机制。方法采用正交设计，以人乳腺癌细胞MCF-7为研究对象，人乳腺正常上皮细胞MCF-10A作为对照，应用CCK-8法，以细胞生长抑制率为指标优选丹参-人参活性组分的最佳配伍；结合实时细胞分析技术验证最佳配伍对细胞的增殖抑制作用；采用高内涵细胞分析技术检测最佳配伍对细胞凋亡的影响。结果优选出丹参-人参抗乳腺癌的最佳配伍为：丹参总酚酸、人参总皂苷和人参多糖，其配比是5、10、5 mg·L-1；实时细胞分析技术检测结果表明，最佳配伍对乳腺癌细胞MCF-7增殖抑制作用明显，对正常乳腺细胞MCF-10A的增殖抑制作用不明显；高内涵荧光细胞分析检测最佳配伍的Hoechst、Annexin V、PI染色荧光，与对照组相比，诱导凋亡作用对于MCF-7细胞差异有显著性(P<0．01)，对于MCF-10A细胞差异无显著性(P>0．05)。结论丹参-人参活性组分的最佳配伍对乳腺癌MCF-7细胞有较强的增殖抑制和诱导凋亡作用，而对正常乳腺MCF-10A细胞无明显影响，具有选择性抗MCF-7乳腺癌的作用。
목적：우선단삼-인삼활성조분항유선암적최가배오，초보연구기작용궤제。방법채용정교설계，이인유선암세포MCF-7위연구대상，인유선정상상피세포MCF-10A작위대조，응용CCK-8법，이세포생장억제솔위지표우선단삼-인삼활성조분적최가배오；결합실시세포분석기술험증최가배오대세포적증식억제작용；채용고내함세포분석기술검측최가배오대세포조망적영향。결과우선출단삼-인삼항유선암적최가배오위：단삼총분산、인삼총조감화인삼다당，기배비시5、10、5 mg·L-1；실시세포분석기술검측결과표명，최가배오대유선암세포MCF-7증식억제작용명현，대정상유선세포MCF-10A적증식억제작용불명현；고내함형광세포분석검측최가배오적Hoechst、Annexin V、PI염색형광，여대조조상비，유도조망작용대우MCF-7세포차이유현저성(P<0．01)，대우MCF-10A세포차이무현저성(P>0．05)。결론단삼-인삼활성조분적최가배오대유선암MCF-7세포유교강적증식억제화유도조망작용，이대정상유선MCF-10A세포무명현영향，구유선택성항MCF-7유선암적작용。
Aim To optimize the most effective compo-nent formula from the active ingredients of Salvia Milti-orrhiza and Panax Ginseng through the orthogonal de-sign method to resist breast cancer, and to reveal its antitumor mechanism in MCF-7 cells. Methods The human breast cancer cells MCF-7 were employed as the research object and the normal breast epithelial cells MCF-10A were used as control,optimizing the most ef-fective component formula from the active ingredients of Salvia Miltiorrhiza and Panax Ginseng by using CCK-8 assay and orthogonal design method; real-time cell a-nalysis was used to monitor the best combination formu-la on cell proliferation, and high content screening was used to detect the best combination drug on cell apop-tosis. Results The best combination of the salvianolic acids, saponins of Panax Ginseng and ginseng polysac-charides that were screened out were 5 , 10 , 5 mg · L-1 . Compared with control group, the treatment group had effective response inhibiting the proliferation on MCF-7 cells, but those effects were weaker on MCF-10A cells through real-time cell analysis. Ho-echst, Annexin V, PI staining fluorescence showed no significant difference ( P >0. 05 ) on MCF-10 A cells compared with the control group,but there was signifi-cant difference ( P <0. 01 ) on MCF-7 cells by HCS. Conclusions The most effective component formula from the active ingredients of Salvia Miltiorrhiza and Panax Ginseng have a strong inhibition of proliferation and induction of apoptosis to resist breast cancer with selection, and there is no significant difference in MCF-10A cells.