CAJ | 학술논문

目的：探讨CD44单抗A3D8对急性早幼粒细胞白血病细胞株NB4细胞IL-3Rα及其下游PI3K/Akt信号通路的调节。方法以NB4细胞为研究对象,以同型抗体IgG1为阴性对照,以A3D8为实验组,A3D8诱导NB4细胞分化凋亡过程中,real-time RT-PCR 方法检测IL-3Rα基因转录水平,运用Western blot 法检测IL-3Rα及下游PI3K/Akt信号通路中重要信号分子,随后将 PI3K/Akt 信号通路抑制剂LY294002与A3D8联合,观察二者联合对 NB4细胞 PI3K/Akt信号通路的影响。结果 A3D8诱导NB4细胞分化凋亡过程,可明显下调NB4细胞中IL-3Rα mRNA和蛋白表达水平,抑制PI3K/Akt信号通路；LY294002可增强A3D8对NB4细胞增殖和凋亡的抑制作用,并可进一步抑制PI3K/Akt信号通路。结论 A3D8可抑制NB4细胞IL-3Rα的转录水平和蛋白表达水平,并抑制其下游PI3K/Akt信号通路。
목적：탐토CD44단항A3D8대급성조유립세포백혈병세포주NB4세포IL-3Rα급기하유PI3K/Akt신호통로적조절。방법이NB4세포위연구대상,이동형항체IgG1위음성대조,이A3D8위실험조,A3D8유도NB4세포분화조망과정중,real-time RT-PCR 방법검측IL-3Rα기인전록수평,운용Western blot 법검측IL-3Rα급하유PI3K/Akt신호통로중중요신호분자,수후장 PI3K/Akt 신호통로억제제LY294002여A3D8연합,관찰이자연합대 NB4세포 PI3K/Akt신호통로적영향。결과 A3D8유도NB4세포분화조망과정,가명현하조NB4세포중IL-3Rα mRNA화단백표체수평,억제PI3K/Akt신호통로；LY294002가증강A3D8대NB4세포증식화조망적억제작용,병가진일보억제PI3K/Akt신호통로。결론 A3D8가억제NB4세포IL-3Rα적전록수평화단백표체수평,병억제기하유PI3K/Akt신호통로。
Aim To investigate the effect of CD44 anti-body-A3 D8 on the expression of IL-3 Rα and down-stream PI3K/Akt in NB4 cells. Methods The ex-pression of IL-3 Rα mRNA was detected by real-time quantitative RT-PCR, the IL-3Rα protein expression and changes of PI3 K/Akt signal pathway in NB4 cells treated with A3D8 were analyzed by Western blot. An-nexin-V-FITC/PI double staining flow cytometry was u-tilized to detect the apoptotic cells. The inhibitor of PI3 K/Akt signaling LY294002 combined with A3 D8 was used to inhibit the PI3K/Akt in NB4 cells. Re-sults After treated with A3 D8 , both the transcription-al level and translational level of IL-3 Rα were remark-ably reduced, and the PI3K/Akt pathway was inhibi-ted. LY294002 improved the inhibitory and apoptotic effects of A3D8 on NB4 cells. Conclusion CD44 antibody A3 D8 can downregulate the expression of IL-3Rα and inhibit the downstream PI3K/Akt pathway.