CAJ | 학술논문

基于手性配体交换机理，研究了以手性离子液体1-乙基-3-甲基咪唑 L-乳酸盐（[ EMIM][ L-Lac]）为手性配体拆分色氨酸对映体（Trys）、苯丙氨酸对映体（ Phes）和酪氨酸对映体（ Tyrs）的方法。实验考察了背景电解质和中心离子种类、手性配体与中心离子的浓度及比例、运行缓冲液 pH 等因素对 Trys、Phes 和 Tyrs 手性拆分的影响。研究发现，当运行缓冲液为40.0 mmol / L[EMIM][ L-Lac]、20.0 mmol / L 氯化铜（ pH 4.5）时，3对对映体均能得到良好的手性拆分。为了验证[EMIM][L-Lac]的良好性能，实验进一步将 L-乳酸（L-Lac）作为手性配体用于 Trys的手性拆分。对比实验发现，单独使用 L-乳酸，DL-Try 只能得到部分拆分，加入离子液体1-乙基-3-甲基咪唑醋酸盐（EMIM-Ace）后，DL-Try 分离情况得到了很大改善，但出峰时间延长至30 min 以上。而使用[ EMIM][ L-Lac]时，Trys 的出峰时间均在15 min 以内。实验最后还对手性拆分机理做了进一步讨论。实验结果表明：在 EMIM-Ace 辅助 L-Lac 体系中，EMIM-Ace 仅被用于抑制电渗流，并未参与手性配体交换反应；而在[ EMIM][ L-Lac]体系中，参与手性配体交换反应的主要是未解离的[EMIM][L-Lac]，而不是解离后的 L-乳酸根离子形成的 L-乳酸。
기우수성배체교환궤리，연구료이수성리자액체1-을기-3-갑기미서 L-유산염（[ EMIM][ L-Lac]）위수성배체탁분색안산대영체（Trys）、분병안산대영체（ Phes）화락안산대영체（ Tyrs）적방법。실험고찰료배경전해질화중심리자충류、수성배체여중심리자적농도급비례、운행완충액 pH 등인소대 Trys、Phes 화 Tyrs 수성탁분적영향。연구발현，당운행완충액위40.0 mmol / L[EMIM][ L-Lac]、20.0 mmol / L 록화동（ pH 4.5）시，3대대영체균능득도량호적수성탁분。위료험증[EMIM][L-Lac]적량호성능，실험진일보장 L-유산（L-Lac）작위수성배체용우 Trys적수성탁분。대비실험발현，단독사용 L-유산，DL-Try 지능득도부분탁분，가입리자액체1-을기-3-갑기미서작산염（EMIM-Ace）후，DL-Try 분리정황득도료흔대개선，단출봉시간연장지30 min 이상。이사용[ EMIM][ L-Lac]시，Trys 적출봉시간균재15 min 이내。실험최후환대수성탁분궤리주료진일보토론。실험결과표명：재 EMIM-Ace 보조 L-Lac 체계중，EMIM-Ace 부피용우억제전삼류，병미삼여수성배체교환반응；이재[ EMIM][ L-Lac]체계중，삼여수성배체교환반응적주요시미해리적[EMIM][L-Lac]，이불시해리후적 L-유산근리자형성적 L-유산。
Enantioseparation of tryptophan enantiomers( Trys),tyrosine enantiomers( Tyrs) and phenylamine enantiomers(Phes)by chiral ligand exchange capillary electrophoresis(CLE-CE)using 1-ethyl-3-methyl imidazole L-lactate([ EMIM][ L-Lac]) as the chiral ligand was studied. The effects of background electrolytes and central ions,the molar ratio and concentra-tion of the chiral ligand and central ion,running buffer solution pH on the enantioseparation of Trys,Phes and Tyrs were investigated. Good enantioseparation of three pairs of enantiomers were obtained with 40. 0 mmol / L[EMIM][L-Lac]and 20. 0 mmol / L cupric chloride(pH 4. 5). To validate the good behavior of[ EMIM][ L-Lac],L-lactate( L-Lac)was further used as the chiral ligand for the enantioseparation of Trys. The contrast experiment showed that Trys could only be partially enantioseparated by L-Lac. Moreover,the enantioseparation was improved by adding 1-ethyl-3-methyl imidazole acetate( EMIM-Ace)which markedly prolonged the migra-tion time of Trys to more than 30 min. In contrast,the migration time of Trys was obtained in 15 min by[ EMIM][ L-Lac]. Finally,the enantioseparation mechanism was further discussed. The experimental results showed that in the EMIM-Ace assisting L-Lac system,EMIM-Ace was only used to suppress the electroosmotic flow and was not involved in the chiral ligand exchange reaction,while in the[EMIM][L-Lac]system,the chiral ligand taking part in the re-action was mainly unionized[EMIM][L-Lac]rather than L-Lac formed by ionized L-Lac ions.