CAJ | 학술논문

目的：探讨黄芪甲苷对Th2型淋巴细胞株( D10. G4.1,D10)体外的干预作用。方法体外培养D10细胞,采用ConA作为刺激剂,分别予以2、4、8×10-5 mol · L-1剂量黄芪甲苷体外干预,并用地塞米松作为对照。 CCK-8法检测药物对D10细胞的增殖影响；流式细胞术检测药物对D10细胞凋亡和周期的影响；ELISA法检测药物对细胞培养上清IL-4、IL-5、IL-13及TNF-α水平的影响；Western blot法检测D10细胞gata-3表达水平。结果黄芪甲苷各剂量组对D10细胞增殖及凋亡均无明显影响( P>0．05)；但黄芪甲苷高剂量组IL-13与gata-3表达水平明显下降(P<0．01),而低剂量组TNF-α明显下降(P<0．01)。结论黄芪甲苷体外具有抑制Th2反应的作用,但与药物细胞毒性或诱导凋亡无关。
목적：탐토황기갑감대Th2형림파세포주( D10. G4.1,D10)체외적간예작용。방법체외배양D10세포,채용ConA작위자격제,분별여이2、4、8×10-5 mol · L-1제량황기갑감체외간예,병용지새미송작위대조。 CCK-8법검측약물대D10세포적증식영향；류식세포술검측약물대D10세포조망화주기적영향；ELISA법검측약물대세포배양상청IL-4、IL-5、IL-13급TNF-α수평적영향；Western blot법검측D10세포gata-3표체수평。결과황기갑감각제량조대D10세포증식급조망균무명현영향( P>0．05)；단황기갑감고제량조IL-13여gata-3표체수평명현하강(P<0．01),이저제량조TNF-α명현하강(P<0．01)。결론황기갑감체외구유억제Th2반응적작용,단여약물세포독성혹유도조망무관。
Aim To evaluate the effects of astragalo-sideⅣon Th2 lymphocyte D10. G4. 1 (D10) in vitro. Methods Cells were cultured in vitro according to ATCC recommendations and were treated with astraga-loside IV at dose of 2, 4, 8 × 10-5 mol·L-1 or dexam-ethasone ( Dex) in the present of ConA. Cell viability was determined by cell counting kit-8 assay. Apoptosis and cycle distribution of D10 were evaluated by flow cytometry. Th2 cytokines in the culture supernatant of D10 were evaluated by ELISA. Th2 transcription factor GATA-3 expression in D10 cells was determined by Western blot. Results Astragaloside IV at dose of 2, 4,8 × 10 -5 mol·L-1 have no significant effect on cell viability or apoptosis of D10 ( P>0. 05 ) . Astragaloside IV at dose of 8 × 10-5 mol · L-1 markedly suppressed the level of IL-13 in the culture supernatant ( P <0. 01 ) , and the expression of GATA-3 ( P <0. 05 ) , and Astragaloside IV at dose of 2 × 10 -5 mol·L-1 ob-viously inhibited the level of TNF-α ( P < 0. 01 ) . Conclusion Astragaloside IV has immunomodulatory properties on Th2 response in vitro,which is not associ-ated with cytotoxic effect or apoptosis.