CAJ | 학술논문

目的探讨甘草酸二铵（ DG）对百草枯中毒（ PQ）致急性肺损伤（ ALI）大鼠肺Toll样受体4（TLR-4） mRNA、NF-κB mRNA表达及对血清白细胞介素-6（IL-6）、白细胞介素-17（IL-17）浓度的影响。方法将50只SD大鼠采用随机数字表法分为PQ组（n＝20）、DG治疗组（n＝20）和正常对照组（S组，n＝10），PQ组和DG治疗组予百草枯100 mg/kg灌胃1次，DG组于灌胃后立即腹腔注射DG 50 mg/kg（1次/d），S组和PQ组注射等剂量的生理盐水，观察至48 h。取肺组织检测肺湿干质量比（ W/D）；采用RT-PCR法检测肺组织TLR-4 mRNA、NF-κB mRNA的表达情况；ELISA法测定血清IL-6、IL-17浓度。另选择健康SD大鼠50只，分组及各组处置方法同上，观察其7 d内死亡率。结果与S组比较，PQ组W/D、TLR-4 mRNA、NF-κB mRNA表达均明显升高（P＜0．01），血清IL-6、IL-17亦升高（P＜0．01）；DG组干预后，W/D降低， TLR-4 mRNA、NF-kB mRN、AIL-6及IL-17有所降低，与PQ组比较差异有统计学意义（ P＜0.01或P＜0．05）。 PQ组大鼠7 d死亡18只（死亡率90％），DG组死亡8只（死亡率40％），NS组无死亡（死亡率0）。结论甘草酸二铵可抑制PQ致ALI大鼠肺组织TLR-4 mRNA、NF-κB mRNA及血清IL-6、IL-17浓度的过度表达，从而减轻肺损伤程度。
목적탐토감초산이안（ DG）대백초고중독（ PQ）치급성폐손상（ ALI）대서폐Toll양수체4（TLR-4） mRNA、NF-κB mRNA표체급대혈청백세포개소-6（IL-6）、백세포개소-17（IL-17）농도적영향。방법장50지SD대서채용수궤수자표법분위PQ조（n＝20）、DG치료조（n＝20）화정상대조조（S조，n＝10），PQ조화DG치료조여백초고100 mg/kg관위1차，DG조우관위후립즉복강주사DG 50 mg/kg（1차/d），S조화PQ조주사등제량적생리염수，관찰지48 h。취폐조직검측폐습간질량비（ W/D）；채용RT-PCR법검측폐조직TLR-4 mRNA、NF-κB mRNA적표체정황；ELISA법측정혈청IL-6、IL-17농도。령선택건강SD대서50지，분조급각조처치방법동상，관찰기7 d내사망솔。결과여S조비교，PQ조W/D、TLR-4 mRNA、NF-κB mRNA표체균명현승고（P＜0．01），혈청IL-6、IL-17역승고（P＜0．01）；DG조간예후，W/D강저， TLR-4 mRNA、NF-kB mRN、AIL-6급IL-17유소강저，여PQ조비교차이유통계학의의（ P＜0.01혹P＜0．05）。 PQ조대서7 d사망18지（사망솔90％），DG조사망8지（사망솔40％），NS조무사망（사망솔0）。결론감초산이안가억제PQ치ALI대서폐조직TLR-4 mRNA、NF-κB mRNA급혈청IL-6、IL-17농도적과도표체，종이감경폐손상정도。
Objective To evaluate the effect of diammonium glycyrrhizinate ( DG ) on paraquat-induced acute lung injury in rats.Methods Fifty SD rats were randomly divided into control group ( NS ) , PQ group and DG group.The rats in PQ group and DG group were intragastriced administration with paraquat (100 mg/kg) .The rats in DG group were intragastriced administration with DG (50 mg/kg) once per day after paraquat injection.Pulmonary lung wet to dry weight ratio was determined;the lung tissue HE staining was used to evaluate the pulmonary tissue injury;to detect the expression of lung tissue TLR -4 mRNA and NF -κB mRNA RT -PCR method was used.The concentration of IL-6 and IL-17 in serum were determined by ELISA.Results Compared with the control group, the lung tissue's wet/dry weight ratio, expression of lung tissue TLR-4 mRNA, NF-κB mRNA, IL-6 and IL -17 in serum increased significantly and comparison between groups showed statistical significance (P<0.01 or P<0.05).7 day mortality was 90% in PQ group, 40% in DG group, and no death in NS group.Conclusion DG can attenuate the acute lung injury induced by paraquat poisoning in rats, the mechanism may be related to the decrease of TLR -4 and NF -κB mRNA expression.