中国急救医学
中國急救醫學
중국급구의학
CHINESE JOURNAL OF CRITICAL CARE MEDICINE
2014年
11期
1017-1021
,共5页
吴杨%侯宏伟%杨丛林%陆洋%辛显峰%于洋%刘家丰
吳楊%侯宏偉%楊叢林%陸洋%辛顯峰%于洋%劉傢豐
오양%후굉위%양총림%륙양%신현봉%우양%류가봉
α2-Heremans-Schimid糖蛋白( AHSG)%基因多态性%脑梗死
α2-Heremans-Schimid糖蛋白( AHSG)%基因多態性%腦梗死
α2-Heremans-Schimid당단백( AHSG)%기인다태성%뇌경사
α2-Heremans-Schimid glycoprotein( AHSG)%Polymorphism%Cerebral infarction
目的探讨α2-Heremans-Schimid -糖蛋白( AHSG)的5个单核苷酸多态性位点( rs4917、rs4918、rs1071592、rs2070633、rs2070635)与脑梗死的相关性。方法采用直接测序法对200例脑梗死患者及100例健康对照者进行5个多态性位点检测,检测结果以 Hardy -Weinberg平衡检验观察样本是否具有群体代表性。χ2检验对计量资料、基因型和等位基因频率分布进行检验。基因型和等位基因风险率采用相对危险度( OR)表示。结果 AHSG基因的rs4917位点的CC、CT、TT基因型在脑梗死组和对照组间比较差异无统计学意义(χ2=5.243,P=0.079)。CC基因型频率在脑梗死组显著高于对照组(χ2=5.230,P=0.027), CC基因型患脑梗死风险较CT+TT基因型高( OR=1.758,95%CI 1.082~2.856)。 C等位基因频率在两组间比较差异有统计学意义(χ2=4.758,P=0.032,OR=1.492,95%CI 1.040~2.139)。 rs4918位点的CC、CG、GG基因型在脑梗死组和对照组间比较差异有统计学意义(χ2=7.335,P=0.024)。 GG基因型频率在脑梗死组显著高于对照组(χ2=5.055,P=0.028),GG基因型患脑梗死风险较CG+CC基因型高(OR=2.965,95%CI 1.106~7.953)。 G等位基因在脑梗死组中的频率高于对照组,但差异无统计学意义(χ2=0.408,P=0.566)。 rs1071592位点的CC、AC、AA基因型在脑梗死组和对照组间比较差异无统计学意义(χ2=5.375,P=0.065)。 AA基因型频率在脑梗死组显著高于对照组(χ2=4.615,P=0.037),AA 基因型患脑梗死风险较 AC +CC 基因型高( OR=3.593,95%CI 1.041~12.394)。 A等位基因在脑梗死组中的频率高于对照组,但差异无统计学意义(χ2=0.942,P=0.351,OR=1.229,95%CI 0.810~1.864)。两组rs2070633、rs2070635位点基因型及等位基因频率分布及相对风险分析比较差异无统计学意义(P>0.05)。结论 AHSG基因rs4917位点CC基因型与C等位基因、rs4918位点GG基因型、rs1071592位点AA基因型可能增加人群患脑梗死的风险,其中rs4917位点C等位基因可能是脑梗死的易感基因。
目的探討α2-Heremans-Schimid -糖蛋白( AHSG)的5箇單覈苷痠多態性位點( rs4917、rs4918、rs1071592、rs2070633、rs2070635)與腦梗死的相關性。方法採用直接測序法對200例腦梗死患者及100例健康對照者進行5箇多態性位點檢測,檢測結果以 Hardy -Weinberg平衡檢驗觀察樣本是否具有群體代錶性。χ2檢驗對計量資料、基因型和等位基因頻率分佈進行檢驗。基因型和等位基因風險率採用相對危險度( OR)錶示。結果 AHSG基因的rs4917位點的CC、CT、TT基因型在腦梗死組和對照組間比較差異無統計學意義(χ2=5.243,P=0.079)。CC基因型頻率在腦梗死組顯著高于對照組(χ2=5.230,P=0.027), CC基因型患腦梗死風險較CT+TT基因型高( OR=1.758,95%CI 1.082~2.856)。 C等位基因頻率在兩組間比較差異有統計學意義(χ2=4.758,P=0.032,OR=1.492,95%CI 1.040~2.139)。 rs4918位點的CC、CG、GG基因型在腦梗死組和對照組間比較差異有統計學意義(χ2=7.335,P=0.024)。 GG基因型頻率在腦梗死組顯著高于對照組(χ2=5.055,P=0.028),GG基因型患腦梗死風險較CG+CC基因型高(OR=2.965,95%CI 1.106~7.953)。 G等位基因在腦梗死組中的頻率高于對照組,但差異無統計學意義(χ2=0.408,P=0.566)。 rs1071592位點的CC、AC、AA基因型在腦梗死組和對照組間比較差異無統計學意義(χ2=5.375,P=0.065)。 AA基因型頻率在腦梗死組顯著高于對照組(χ2=4.615,P=0.037),AA 基因型患腦梗死風險較 AC +CC 基因型高( OR=3.593,95%CI 1.041~12.394)。 A等位基因在腦梗死組中的頻率高于對照組,但差異無統計學意義(χ2=0.942,P=0.351,OR=1.229,95%CI 0.810~1.864)。兩組rs2070633、rs2070635位點基因型及等位基因頻率分佈及相對風險分析比較差異無統計學意義(P>0.05)。結論 AHSG基因rs4917位點CC基因型與C等位基因、rs4918位點GG基因型、rs1071592位點AA基因型可能增加人群患腦梗死的風險,其中rs4917位點C等位基因可能是腦梗死的易感基因。
목적탐토α2-Heremans-Schimid -당단백( AHSG)적5개단핵감산다태성위점( rs4917、rs4918、rs1071592、rs2070633、rs2070635)여뇌경사적상관성。방법채용직접측서법대200례뇌경사환자급100례건강대조자진행5개다태성위점검측,검측결과이 Hardy -Weinberg평형검험관찰양본시부구유군체대표성。χ2검험대계량자료、기인형화등위기인빈솔분포진행검험。기인형화등위기인풍험솔채용상대위험도( OR)표시。결과 AHSG기인적rs4917위점적CC、CT、TT기인형재뇌경사조화대조조간비교차이무통계학의의(χ2=5.243,P=0.079)。CC기인형빈솔재뇌경사조현저고우대조조(χ2=5.230,P=0.027), CC기인형환뇌경사풍험교CT+TT기인형고( OR=1.758,95%CI 1.082~2.856)。 C등위기인빈솔재량조간비교차이유통계학의의(χ2=4.758,P=0.032,OR=1.492,95%CI 1.040~2.139)。 rs4918위점적CC、CG、GG기인형재뇌경사조화대조조간비교차이유통계학의의(χ2=7.335,P=0.024)。 GG기인형빈솔재뇌경사조현저고우대조조(χ2=5.055,P=0.028),GG기인형환뇌경사풍험교CG+CC기인형고(OR=2.965,95%CI 1.106~7.953)。 G등위기인재뇌경사조중적빈솔고우대조조,단차이무통계학의의(χ2=0.408,P=0.566)。 rs1071592위점적CC、AC、AA기인형재뇌경사조화대조조간비교차이무통계학의의(χ2=5.375,P=0.065)。 AA기인형빈솔재뇌경사조현저고우대조조(χ2=4.615,P=0.037),AA 기인형환뇌경사풍험교 AC +CC 기인형고( OR=3.593,95%CI 1.041~12.394)。 A등위기인재뇌경사조중적빈솔고우대조조,단차이무통계학의의(χ2=0.942,P=0.351,OR=1.229,95%CI 0.810~1.864)。량조rs2070633、rs2070635위점기인형급등위기인빈솔분포급상대풍험분석비교차이무통계학의의(P>0.05)。결론 AHSG기인rs4917위점CC기인형여C등위기인、rs4918위점GG기인형、rs1071592위점AA기인형가능증가인군환뇌경사적풍험,기중rs4917위점C등위기인가능시뇌경사적역감기인。
Objective To investigate the relationship between five single -nucleotide polymorphism (SNP) ofα2-Heremans-Schimid glycoprotein (AHSG) and cerebral infarction(CI). Methods Five gene polymorphisms were detected for 200 patients of CI and 100 healthy controls using direct sequencing method.The χ2 test was adopted to check the difference in genotype and allele frequency distribution between the cases and the controls, the accordance with Hardy-Weinberg equilibrium in the group, and genotype and allele risk rate were showed with odds ratio ( OR) .Results There were no significant differences(χ2 =5.243,P=0.079) between CI and healthy control in CC, CT, TT genotypes of rs4917 of AHSG gene.The frequency of CC genotype in CI group was significantly higher than in the control group(χ2 =5.230,P=0.027).And the diseased risk of CI in genotype CC was higher than CT+TT (OR=1.758,95%CI 1.082~2.856).The frequency of C allele between CI and healthy control had significant differences (χ2 =4.758,P=0.032,OR=1.492,95%CI 1.040~2.139) .The frequencies of CC, CG, GG of rs4018 of AHSG gene had significant difference between two groups(χ2 =7.335,P=0.024).Compared with genotype CG+CC, the frequency of genotype GG in CI was higher than in the control group(χ2 =5.055,P=0.028) .And the diseased risk of CI in genotype GG was higher than CG+CC (OR=2.965,95%CI 1.106 ~7.953).The frequency of G allele in CI was higher than in the control group, which had no significant differences(χ2 =0.408,P=0.566) .The frequencies of CC, AC, AA of 1071592 of AHSG gene had no significant difference between two groups(χ2 =5.375,P=0.065).Compared with genotype AC +CC, the frequency of genotype AA in CI was significantly higher than in the control group(χ2 =4.615,P=0.037), and the diseased risk of CI in genotype AA was higher than AC+CC (OR=3.593,95%CI 1.041~12.394). The frequency of A allele in CI was higher than in the control group, but which had no significant differences (χ2 =0.942,P=0.351,OR=1.229,95%CI 0.810 ~1.864).There were no significant differences among rs2070633、rs2070635 between two groups. Conclusion AHSG gene rs4917 genotype CC and C allele, rs4918 genotype GG, rs1071592 genotype AA might be a risk factor for increasing the diseased rate of cerebral infarction in population, which rs4917 C allele might be predisposing genes for cerebral infarction.