中华肝脏外科手术学电子杂志
中華肝髒外科手術學電子雜誌
중화간장외과수술학전자잡지
CHINESE JOURNAL OF HEPATIC SURGERY(ELECTRONIC EDITION)
2014年
5期
287-290
,共4页
黄小慧%许颖君%施巧素%张琪%易慧敏
黃小慧%許穎君%施巧素%張琪%易慧敏
황소혜%허영군%시교소%장기%역혜민
肝移植%感染%免疫测定%T 淋巴细胞亚群%三磷酸腺苷
肝移植%感染%免疫測定%T 淋巴細胞亞群%三燐痠腺苷
간이식%감염%면역측정%T 림파세포아군%삼린산선감
Liver transplantation%Infection%Immunoassay%T-lymphocyte subsets%Adenosine triphosphate
目的探讨 Cylex ImmuKnow 免疫细胞功能检测在肝移植术后感染诊断中的价值。方法前瞻性研究对象为2010年7月至2012年12月在中山大学附属第三医院肝移植中心接受同种异体原位肝移植术的48例患者。所有患者均签署知情同意书，符合医学伦理学规定。根据肝移植术后有否临床感染将患者分为感染组（14例）、非感染组（34例），另设健康对照组（对照组，20例）。其中感染组男11例，女3例，平均年龄（47±10）岁；非感染组男29例，女5例，年龄（45±9）岁；对照组男10例，女10例，年龄（42±10）岁。患者肝移植术后7 d 和对照组入组时，抽取外周静脉血2 ml，应用 Cylex ImmuKnow 免疫细胞功能测定试剂盒检测分化群（CD）4+淋巴细胞的三磷酸腺苷（ATP）含量，检测数据比较采用 t 检验。结果感染组、非感染组和对照组的 ATP 含量分别为（178±58）、（345±62）、（467±84）mg/L，感染组 ATP 含量明显低于非感染组（t=-4.6，P<0.05）。Cylex ImmuKnow 免疫细胞功能检测诊断肝移植术后感染的灵敏度为0.79，特异度为0.85；阳性预测值为0.69，阴性预测值为0.91。结论 Cylex ImmuKnow 免疫细胞功能检测能够简便、快速检测患者免疫细胞功能水平，对肝移植术后感染具有一定的监测和诊断价值。
目的探討 Cylex ImmuKnow 免疫細胞功能檢測在肝移植術後感染診斷中的價值。方法前瞻性研究對象為2010年7月至2012年12月在中山大學附屬第三醫院肝移植中心接受同種異體原位肝移植術的48例患者。所有患者均籤署知情同意書，符閤醫學倫理學規定。根據肝移植術後有否臨床感染將患者分為感染組（14例）、非感染組（34例），另設健康對照組（對照組，20例）。其中感染組男11例，女3例，平均年齡（47±10）歲；非感染組男29例，女5例，年齡（45±9）歲；對照組男10例，女10例，年齡（42±10）歲。患者肝移植術後7 d 和對照組入組時，抽取外週靜脈血2 ml，應用 Cylex ImmuKnow 免疫細胞功能測定試劑盒檢測分化群（CD）4+淋巴細胞的三燐痠腺苷（ATP）含量，檢測數據比較採用 t 檢驗。結果感染組、非感染組和對照組的 ATP 含量分彆為（178±58）、（345±62）、（467±84）mg/L，感染組 ATP 含量明顯低于非感染組（t=-4.6，P<0.05）。Cylex ImmuKnow 免疫細胞功能檢測診斷肝移植術後感染的靈敏度為0.79，特異度為0.85；暘性預測值為0.69，陰性預測值為0.91。結論 Cylex ImmuKnow 免疫細胞功能檢測能夠簡便、快速檢測患者免疫細胞功能水平，對肝移植術後感染具有一定的鑑測和診斷價值。
목적탐토 Cylex ImmuKnow 면역세포공능검측재간이식술후감염진단중적개치。방법전첨성연구대상위2010년7월지2012년12월재중산대학부속제삼의원간이식중심접수동충이체원위간이식술적48례환자。소유환자균첨서지정동의서，부합의학윤리학규정。근거간이식술후유부림상감염장환자분위감염조（14례）、비감염조（34례），령설건강대조조（대조조，20례）。기중감염조남11례，녀3례，평균년령（47±10）세；비감염조남29례，녀5례，년령（45±9）세；대조조남10례，녀10례，년령（42±10）세。환자간이식술후7 d 화대조조입조시，추취외주정맥혈2 ml，응용 Cylex ImmuKnow 면역세포공능측정시제합검측분화군（CD）4+림파세포적삼린산선감（ATP）함량，검측수거비교채용 t 검험。결과감염조、비감염조화대조조적 ATP 함량분별위（178±58）、（345±62）、（467±84）mg/L，감염조 ATP 함량명현저우비감염조（t=-4.6，P<0.05）。Cylex ImmuKnow 면역세포공능검측진단간이식술후감염적령민도위0.79，특이도위0.85；양성예측치위0.69，음성예측치위0.91。결론 Cylex ImmuKnow 면역세포공능검측능구간편、쾌속검측환자면역세포공능수평，대간이식술후감염구유일정적감측화진단개치。
Objective To investigate the value of Cylex ImmuKnow immune cell function assay in the diagnosis of infection after liver transplantation. Methods The objects of this prospective study were 48 patients undergoing orthotopic liver transplantation (OLT) in Liver Transplantation Center, the Third Affiliated Hospital of Sun Yat-sen University from July 2010 to December 2012. The informed consents of all patients were obtained and the ethical committee approval was received. According to whether the patients suffered clinical infection or not after OLT, they were divided into two groups:infection group (n=14) and non-infection group (n=34). Another 20 healthy people were enrolled as the control group. There were 11 males and 3 females in the infection group with a mean age of (47±10) years old. There were 29 males and 5 females in the non-infection group with a mean age of (45±9) years old. There were 10 males and 10 females in the control group with a mean age of (42±10) years old. Peripheral venous blood 2 ml was collected from the patients on the 7th day after OLT or from the healthy people when enrolled in control group. The adenosine triphosphate (ATP) level in cluster of differentiation (CD) 4+ lymphocyte was detected using Cylex ImmuKnow kit and the testing data were compared using t test. Results The ATP level in CD4+ cells was (178±58), (345±62), (467±84) mg/L in infection group, non-infection group and control group respectively. The ATP level in infection group was significantly lower than that in non-infection group (t =-4.6, P<0.05). The sensitivity of the Cylex ImmuKnow immune cell function assay in the diagnosis of infection after OLT was 0.79, the specificity was 0.85, the positive predictive value was 0.69, and the negative predictive value was 0.91. Conclusions Cylex ImmuKnow immune cell function assay can simply and quickly detect the immune cell function. It plays a role in monitoring and diagnosing the infection after OLT.
