浙江创伤外科
浙江創傷外科
절강창상외과
ZHEJIANG JOURNAL OF TRAUMATIC SURGERY
2014年
5期
694-696,697
,共4页
MCF-7细胞%p21%siRNA抑制%表阿霉素%Caspase家族
MCF-7細胞%p21%siRNA抑製%錶阿黴素%Caspase傢族
MCF-7세포%p21%siRNA억제%표아매소%Caspase가족
MCF-7 cells%p21%siRNA interference%Epirubicin%Caspase family
目的:研究siRNA沉默p21基因表达联合表阿霉素对MCF-7细胞增殖和凋亡的影响及相关作用机制。方法将化学合成的针对p21的siRNA序列转染至MCF-7细胞,将MCF-7细胞分为5组:空白对照组、阴性对照组、RNA抑制组、表阿霉素组、联合组(RNA沉默+表阿霉素)。采用MTT比色法、流式细胞术和分光光度法分别检测MCF-7细胞增殖、细胞凋亡、细胞周期及半胱氨酸天冬氨酸蛋白酶家族成员Caspase-9、Caspase-3和Caspase-6的活化程度。结果 p21 siRNA转染后,相对于表阿霉素单独处理组,联合组细胞死亡率明显升高(P<0.01);Caspase-9、Caspase-3和Caspase-6的活化程度显著升高(P<0.01)。结论 p21靶向RNA抑制联合表阿霉素处理显著促进了MCF-7细胞凋亡,p21可作为乳腺癌基因治疗的后选新靶点。
目的:研究siRNA沉默p21基因錶達聯閤錶阿黴素對MCF-7細胞增殖和凋亡的影響及相關作用機製。方法將化學閤成的針對p21的siRNA序列轉染至MCF-7細胞,將MCF-7細胞分為5組:空白對照組、陰性對照組、RNA抑製組、錶阿黴素組、聯閤組(RNA沉默+錶阿黴素)。採用MTT比色法、流式細胞術和分光光度法分彆檢測MCF-7細胞增殖、細胞凋亡、細胞週期及半胱氨痠天鼕氨痠蛋白酶傢族成員Caspase-9、Caspase-3和Caspase-6的活化程度。結果 p21 siRNA轉染後,相對于錶阿黴素單獨處理組,聯閤組細胞死亡率明顯升高(P<0.01);Caspase-9、Caspase-3和Caspase-6的活化程度顯著升高(P<0.01)。結論 p21靶嚮RNA抑製聯閤錶阿黴素處理顯著促進瞭MCF-7細胞凋亡,p21可作為乳腺癌基因治療的後選新靶點。
목적:연구siRNA침묵p21기인표체연합표아매소대MCF-7세포증식화조망적영향급상관작용궤제。방법장화학합성적침대p21적siRNA서렬전염지MCF-7세포,장MCF-7세포분위5조:공백대조조、음성대조조、RNA억제조、표아매소조、연합조(RNA침묵+표아매소)。채용MTT비색법、류식세포술화분광광도법분별검측MCF-7세포증식、세포조망、세포주기급반광안산천동안산단백매가족성원Caspase-9、Caspase-3화Caspase-6적활화정도。결과 p21 siRNA전염후,상대우표아매소단독처리조,연합조세포사망솔명현승고(P<0.01);Caspase-9、Caspase-3화Caspase-6적활화정도현저승고(P<0.01)。결론 p21파향RNA억제연합표아매소처리현저촉진료MCF-7세포조망,p21가작위유선암기인치료적후선신파점。
Objective To study the influence of siRNA inhibition of p21 combined with EPI treatment on proliferation and apoptosis of MCF-7 cells and its related mechanism. Methods The siRNA sequence targeting p21 which was synthesized by chemical method was transfected into MCF-7 cells. The MCF-7 cells were divided into five groups: blank control group, negative control group, siRNA group, EPI treated group and combination group (siRNA combined with EPI treatment).MTT assay, flow cytometry and spectrophotometry were employed to detect the variation of cell viability, apoptosis and Caspase-9, Caspase-3 and Caspase-6 activity. Results After transfection, compared with the EPI treated group, the cell death rate of the combination group were markedly increased ( P<0.01), and activity of Caspase-9, Caspase-3 and Caspase-6 was obviously in-duced (P<0.01). Conclusion SiRNA combined with EPI treatment can significantly induce cell apoptosis, p21 gene may be a candidate target in the gene therapy of breast cancer.