CAJ | 학술논문

目的：探讨脊髓神经元凋亡在鞘内注射血小板活化因子(PAF)诱发大鼠痛敏中的作用。方法鞘内置管成功的雄性 Sprague-Dawley 大鼠60只,随机分为2组：对照组,30只,鞘内注射人工脑脊液(arti-ficial cerebral spinal fluid,ACSF)10μl；PAF 组,30只,鞘内注射 PAF 10μg,溶解于10μl 人工脑脊液；分别于鞘内给药前1 d、给药后1、3、5、7、14 d 分别测定机械痛阈(PWMT)和热痛阈(PWTL)。取 L4-6脊髓,采用TUNEL 法观察脊髓神经元凋亡。结果鞘内注射血小板活化因子(PAF)可诱发出大鼠机械性触诱发痛和热痛觉过敏。PAF 组术后1 d 脊髓中开始有少量凋亡神经元出现,凋亡指数于术后3 d 开始迅速增加,术后5 d 达峰值,与对照组比较,有显著性差异(P <0.01)。结论鞘内注射 PAF 诱发大鼠触觉异常痛敏和热痛敏,脊髓神经元凋亡可能参与了鞘内注射 PAF 大鼠痛敏的形成。
목적：탐토척수신경원조망재초내주사혈소판활화인자(PAF)유발대서통민중적작용。방법초내치관성공적웅성 Sprague-Dawley 대서60지,수궤분위2조：대조조,30지,초내주사인공뇌척액(arti-ficial cerebral spinal fluid,ACSF)10μl；PAF 조,30지,초내주사 PAF 10μg,용해우10μl 인공뇌척액；분별우초내급약전1 d、급약후1、3、5、7、14 d 분별측정궤계통역(PWMT)화열통역(PWTL)。취 L4-6척수,채용TUNEL 법관찰척수신경원조망。결과초내주사혈소판활화인자(PAF)가유발출대서궤계성촉유발통화열통각과민。PAF 조술후1 d 척수중개시유소량조망신경원출현,조망지수우술후3 d 개시신속증가,술후5 d 체봉치,여대조조비교,유현저성차이(P <0.01)。결론초내주사 PAF 유발대서촉각이상통민화열통민,척수신경원조망가능삼여료초내주사 PAF 대서통민적형성。
Objective To investigate the roles of neuronal apoptosis in development of tactile allodynia and thermal hyperalgesia induced by platelet-activating factor administered intrathecally.Methods 60 Sprague-Dawley rats with intrathecal PE-10 catheters were randomly divided into two groups:control(artificial cerebral spinal fluid,10 μl)group,PAF (10 μg,dissolved in 10 μl ACSF)group.Paw withdrawal mechanical threshold and thermal latency were measured at-1 ,1 ,3,5,7 and 14 days after surgery .Changes of apoptosis of spinal neuron were observed by TUNEL.Results Intrathecally administered PAF induced development of tactile all-odynia and thermal hyperalgesia rapidly,The expression of spinal neuronal apoptotic index in control group were significantly lower than those in the PAF group(P <0.01).Conclusions Intrathecally administered PAF may induce development of tactile allodynia and thermal hyperalgesia in rats,and this process might be associ-ated with neuronal apoptosis in spinal cord.