CAJ | 학술논문

本研究对湖北恩施烟区青枯病菌（Ralstonia solanacearum）的生理小种、生化型和演化型进行了鉴定，为湖北省烟草青枯病抗性品种的选育和利用提供了理论依据。从湖北恩施地区的8个县（市）采集青枯病感病烟株，通过平板稀释分离和特性性引物检测从中分离鉴定到54个具有致病性的烟草青枯菌菌株。采用注茎接种法将供试菌株分别接种青枯菌6个鉴别寄主，确定所有的菌株均属于生理小种1；基于青枯菌对3种双糖和3种己醇利用能力的检测结果，将54个菌株鉴定为生化型Ⅲ；经青枯菌演化型PCR检测，54个菌株均能扩增得到片段大小分别为280 bp和144 bp的两条特异性条带，这说明全部分离菌株均属于青枯菌演化型Ⅰ型。
본연구대호북은시연구청고병균（Ralstonia solanacearum）적생리소충、생화형화연화형진행료감정，위호북성연초청고병항성품충적선육화이용제공료이론의거。종호북은시지구적8개현（시）채집청고병감병연주，통과평판희석분리화특성성인물검측종중분리감정도54개구유치병성적연초청고균균주。채용주경접충법장공시균주분별접충청고균6개감별기주，학정소유적균주균속우생리소충1；기우청고균대3충쌍당화3충기순이용능력적검측결과，장54개균주감정위생화형Ⅲ；경청고균연화형PCR검측，54개균주균능확증득도편단대소분별위280 bp화144 bp적량조특이성조대，저설명전부분리균주균속우청고균연화형Ⅰ형。
To provide theoretical information for breeding and utilizing bacterial wilt-resistant tobacco cultivars race, biovar and phylotype of Ralstonia solanacearum of tobacco in Hubei province were identified. Diseased plant samples of tobacco bacterial wilt were collected from eight counties of Enshi area in Hubei province, and 54 strains of the pathogen, Ralstonia solanacearum, were isolated from these samples by using the dilution plate isolation method and identified by specific-PCR assay. Based on reactions on six physiological-race hosts, all 54 strains were proved to belong to race 1. On the basis of ability for oxidizing three hexose (lactose, maltose and cellobiose) and three disaccharides (mannitol, sorbitol and dulcite), all strains were identified as biovar III. Phylotype specific multiplex PCR revealved that all 54 isolates, producing both 144 bp and 280 bp amplicons, belonged to R. solanacearum phylotype I.