CAJ | 학술논문

目的：探讨结肠癌组织中液泡型ATP合酶( V-ATPase)表达与结肠癌临床病理特征的关系及其对结肠癌发生发展的意义。方法随机选取20例结肠肿瘤组织和配对的正常组织,通过逆转录-聚合酶链反应( RT-PCR)和实时定量聚合酶链反应( Real-time PCR)检测V-ATPase基因的mRNA表达量,采用免疫组织化EnVinsion法检测V-ATPase基因在68例结肠癌组织及配对的正常癌旁组织中蛋白表达量。结果结肠癌组织及配对正常组织中V-ATPase 基因mRNA平均表达量分别为(5.37±0.44)、(2.03±0.35)(P <0.01)。免疫组织化学结果显示 V-ATPase 在结肠肿瘤组织及配对正常组织中阳性表达率分别为69.1%(47/68)和5.8%(4/68),其阳性表达主要在胞膜和胞质中,V-ATPase过表达与肿瘤分期(P<0.05)、区域淋巴结转移(P=0.044)、远处转移(P=0.049)、脉管浸润(P=0.044)和分化(P<0.001)有关。结论 V-ATPase基因在结肠癌组织中高表达对结肠癌的发生发展起重要作用,可能为结肠癌术后辅助化疗的重要靶点。
목적：탐토결장암조직중액포형ATP합매( V-ATPase)표체여결장암림상병리특정적관계급기대결장암발생발전적의의。방법수궤선취20례결장종류조직화배대적정상조직,통과역전록-취합매련반응( RT-PCR)화실시정량취합매련반응( Real-time PCR)검측V-ATPase기인적mRNA표체량,채용면역조직화EnVinsion법검측V-ATPase기인재68례결장암조직급배대적정상암방조직중단백표체량。결과결장암조직급배대정상조직중V-ATPase 기인mRNA평균표체량분별위(5.37±0.44)、(2.03±0.35)(P <0.01)。면역조직화학결과현시 V-ATPase 재결장종류조직급배대정상조직중양성표체솔분별위69.1%(47/68)화5.8%(4/68),기양성표체주요재포막화포질중,V-ATPase과표체여종류분기(P<0.05)、구역림파결전이(P=0.044)、원처전이(P=0.049)、맥관침윤(P=0.044)화분화(P<0.001)유관。결론 V-ATPase기인재결장암조직중고표체대결장암적발생발전기중요작용,가능위결장암술후보조화료적중요파점。
Objective To explore the expression of V-ATPase in colon cancer and its clinical significance. Methods Detecting the expression of V-ATPase mRNA in 20 paired of colon tumor tissues and normal tissues by using reverse transcription-polymerase chain reaction ( RT-PCR) and real-time quantitative polymerase chain reaction( Real-time PCR) , and testing the expression of V-ATPase protein by immu-nohistochemistry of EnVinsion. Results The expression of V-ATPase mRNA in tumor tissues and its paired normal tissues were (5. 37 ± 0. 44) and (2. 03 ± 0. 35)(P<0. 01). The positive immunohistochemistry of V-ATPase in tumor tissues and its paired normal tissues were 69. 1%(47/68) and 5. 8%(4/68) respectively, and the positive expression were primarily in cytoplasm and cytomembrane. Overexpression of V-ATPase was associated with tumor stage (P<0. 05), lymph node metastasis (P=0. 044), distant metastasis (P=0. 049), vessel in-vasion (P=0. 044) and differentiation (P<0. 001). Conclusion Overexpression of V-ATPase plays a significant role in the carcinogene-sis and the progression of colon cancer, which might be an important postoperative therapeutic target.