临床小儿外科杂志
臨床小兒外科雜誌
림상소인외과잡지
JOURNAL OF CLINICAL FEDIATRIC SURGERY
2014年
5期
422-426,429
,共6页
阑尾炎%血清%组织%儿童
闌尾炎%血清%組織%兒童
란미염%혈청%조직%인동
Appendicitis%Serum%Tissues%Child
目的：探讨Ghrelin在小儿急性阑尾炎血清和组织中的表达情况。方法选择阑尾炎患儿34例，分别于术前（t1）、术后3天（t2）、术后6 d（t3）采集静脉血；选择肠套叠且无明显腹腔感染患儿为对照组（C组），入院时采集静脉血；测定其白细胞计数、CRP、降钙素原和Ghrelin浓度。按照术后病理结果将急性阑尾炎分为单纯性阑尾炎（A1）、急性化脓性阑尾炎并穿孔（A2）和急性坏疽性阑尾炎（A3）。同时收集阑尾炎、肠套叠患儿手术切除阑尾组织，用免疫组化法测定其Ghrelin含量。结果在急性阑尾炎组，A1、A2、A3血清Ghrelin都低于C，A2、A3标本IOD值低于C。t1、t2 Ghrelin浓度明显低于t3，而t1、t2之间无明显差异。结论检测小儿外周血中Ghrelin浓度，对于判断急性阑尾炎的严重程度价值较小，对病程、治疗、预后的评价有一定应用价值。
目的：探討Ghrelin在小兒急性闌尾炎血清和組織中的錶達情況。方法選擇闌尾炎患兒34例，分彆于術前（t1）、術後3天（t2）、術後6 d（t3）採集靜脈血；選擇腸套疊且無明顯腹腔感染患兒為對照組（C組），入院時採集靜脈血；測定其白細胞計數、CRP、降鈣素原和Ghrelin濃度。按照術後病理結果將急性闌尾炎分為單純性闌尾炎（A1）、急性化膿性闌尾炎併穿孔（A2）和急性壞疽性闌尾炎（A3）。同時收集闌尾炎、腸套疊患兒手術切除闌尾組織，用免疫組化法測定其Ghrelin含量。結果在急性闌尾炎組，A1、A2、A3血清Ghrelin都低于C，A2、A3標本IOD值低于C。t1、t2 Ghrelin濃度明顯低于t3，而t1、t2之間無明顯差異。結論檢測小兒外週血中Ghrelin濃度，對于判斷急性闌尾炎的嚴重程度價值較小，對病程、治療、預後的評價有一定應用價值。
목적：탐토Ghrelin재소인급성란미염혈청화조직중적표체정황。방법선택란미염환인34례，분별우술전（t1）、술후3천（t2）、술후6 d（t3）채집정맥혈；선택장투첩차무명현복강감염환인위대조조（C조），입원시채집정맥혈；측정기백세포계수、CRP、강개소원화Ghrelin농도。안조술후병리결과장급성란미염분위단순성란미염（A1）、급성화농성란미염병천공（A2）화급성배저성란미염（A3）。동시수집란미염、장투첩환인수술절제란미조직，용면역조화법측정기Ghrelin함량。결과재급성란미염조，A1、A2、A3혈청Ghrelin도저우C，A2、A3표본IOD치저우C。t1、t2 Ghrelin농도명현저우t3，이t1、t2지간무명현차이。결론검측소인외주혈중Ghrelin농도，대우판단급성란미염적엄중정도개치교소，대병정、치료、예후적평개유일정응용개치。
Objetive To explore the relation between acute appendicitis in children and Ghrelin expres-sion in serum or its appendix tissue. Methods The blood samples of 34 children with appendicitis were col-lected before the operation,three days and six days after the operation respectively;the blood samples of chil-dren with intussusception without obvious evidence of abdominal infection was collected as the Control group;white blood cells,CRP,procalcitonin and Ghrelin concentrations were detected in all these samples.Acute ap-pendicitis was classified into three groups.While the level of Ghrelin in specimens of appendicitis and intussus-ception were detected by immunohistochemistry. Results In the group with acute appendicitis,A1 ,A2 and A3 Serum Ghrelin were lower than the control group.The IOD values of A2 and A3 specimens was lower than the control group.t1 and t2 Ghrelin concentration were significantly lower than t3.There was no significant differences between t1 and t2. Conclusion Ghrelin is of less value in prognosis in the severity of appendici-tis,but it Is of a certain value for the course of disease,treatment and prognosis.
