CAJ | 학술논문

目的：研究促凋亡基因PDCD5在胆道闭锁及胆总管囊肿患儿胆管组织中的表达规律，探讨促凋亡基因PDCD5在胆道闭锁发病机制中的作用。方法分别取18例胆道闭锁（Biliary Atre-sia，BA）和11例胆总管囊肿（Choledochal cyst，CC）患儿的胆管组织，应用蛋白质印迹（westong blot）与荧光实时定量（quantitative real-time PCR，qRT-PCR）的方法分别检测PDCD5在胆道闭锁与胆总管囊肿患儿胆管组织中的表达，并进行定位和定量分析与比较。结果 PDCD5在CC组胆管组织中的CT值为（6．7292±1．169），高于BA组的相应CT值（4．0125±1．0735），P＜0．05。应用2-ΔΔCT计算出CC 与BA中PDCD5基因表达量的比值为1∶6．57。PDCD5在BA组胆管组织中mRNA转录水平明显高于对照组；Western b1 ot结果显示，PDCD5在BA组胆管组织中蛋白表达量明显高于CC组。结论 PDCD5在胆道闭锁患儿胆管组织中的蛋白和基因表达水平均增强。PDCD5可能参与胆道闭锁胆管上皮细胞凋亡，从而参与胆道闭锁的炎性反应，在胆道闭锁的发病机制中发挥重要作用。
목적：연구촉조망기인PDCD5재담도폐쇄급담총관낭종환인담관조직중적표체규률，탐토촉조망기인PDCD5재담도폐쇄발병궤제중적작용。방법분별취18례담도폐쇄（Biliary Atre-sia，BA）화11례담총관낭종（Choledochal cyst，CC）환인적담관조직，응용단백질인적（westong blot）여형광실시정량（quantitative real-time PCR，qRT-PCR）적방법분별검측PDCD5재담도폐쇄여담총관낭종환인담관조직중적표체，병진행정위화정량분석여비교。결과 PDCD5재CC조담관조직중적CT치위（6．7292±1．169），고우BA조적상응CT치（4．0125±1．0735），P＜0．05。응용2-ΔΔCT계산출CC 여BA중PDCD5기인표체량적비치위1∶6．57。PDCD5재BA조담관조직중mRNA전록수평명현고우대조조；Western b1 ot결과현시，PDCD5재BA조담관조직중단백표체량명현고우CC조。결론 PDCD5재담도폐쇄환인담관조직중적단백화기인표체수평균증강。PDCD5가능삼여담도폐쇄담관상피세포조망，종이삼여담도폐쇄적염성반응，재담도폐쇄적발병궤제중발휘중요작용。
Objetive To characterize the expression and significance of PDCD5 gen in bile duct tissues of Biliary Atresia(BA)and Choledochal Cyst(CC). Methods Small pieces of bile duct tissues were obsta-ined from 1 8 patients with BA and 1 5 patients with CC,expression of PDCD5 from Protein level and Gene level was detected by Weston blot and RT-PCR. Results In the BA patients,the Cycle threshold(CT)of PDCD5 in bile duct tissues was(4.0125 ±1.0735),which lower than the CC patients (6.7292 ±1.169)(P<0.05). The ratio of PDCD5 in CC and BA was 1 ∶6.by 2-ΔΔCT.Above all,expression of PDCD5 from Gene level was enhanced in bile duct tissues of BA;And western blotting,result in the BA patients,expression of PDCD5 from Protein level was higher than CC patients. Conclusion Expression of PDCD5 from Protein level and Gene level were enhanced in bile duct tissues of BA.It is Probably that PDCD5 may be involved in the bile duct epithelia cell apoptosis of BA and participate in BA inflammatory reaction,which play an important role in the pathogenesis of BA.