草原与草坪
草原與草坪
초원여초평
GRASSLAND AND TURF
2014年
5期
6-10
,共5页
塔乌库姆冰草%肌动蛋白%基因克隆%序列分析
塔烏庫姆冰草%肌動蛋白%基因剋隆%序列分析
탑오고모빙초%기동단백%기인극륭%서렬분석
Agropyron cristatum cv.Tawukumu%Actin%gene clone%sequence analysis
根据其他植物 Actin 基因的保守序列设计一对简引并性物,以塔乌库姆冰草叶片总 RNA 为模板,采用 RT-PCR 的方法扩增出 Actin 基因片段并连接到载体上,阳性克隆经 PCR 检测后进行测序。结果表明：该片段长约600 bp,编码199个氨基酸,所得序列与 GenBank 中注册的其他植物 Actin 基因序列同源性均在81%以上,与其他肌动蛋白的氨基酸序列同源性达88%。
根據其他植物 Actin 基因的保守序列設計一對簡引併性物,以塔烏庫姆冰草葉片總 RNA 為模闆,採用 RT-PCR 的方法擴增齣 Actin 基因片段併連接到載體上,暘性剋隆經 PCR 檢測後進行測序。結果錶明：該片段長約600 bp,編碼199箇氨基痠,所得序列與 GenBank 中註冊的其他植物 Actin 基因序列同源性均在81%以上,與其他肌動蛋白的氨基痠序列同源性達88%。
근거기타식물 Actin 기인적보수서렬설계일대간인병성물,이탑오고모빙초협편총 RNA 위모판,채용 RT-PCR 적방법확증출 Actin 기인편단병련접도재체상,양성극륭경 PCR 검측후진행측서。결과표명：해편단장약600 bp,편마199개안기산,소득서렬여 GenBank 중주책적기타식물 Actin 기인서렬동원성균재81%이상,여기타기동단백적안기산서렬동원성체88%。
Degenerate primers were designed based on the conserved sequences of the actin gene according to other plants.Total RNA was extracted from the leaves of Agropyron cristatum cv.Tawukumu.Actin gene frag-ment was obtained by reverse transcription polymerase chain reaction (RT-PCR)and cloned into vector.The positive clone identified by PCR was sequenced.The sequencing result revealed that the Actin gene fragment from Agropyron cristatum cv.Tawukumu contains about 600 bp,encoding 1 99 amino acids.Homology compari-son with Actin gene sequences of other plants in the GenBank and with other actins showed that it shared over 81% nucleotide sequence homology and 88% amino acid sequence homology.
