CAJ | 학술논문

目的：检测 RA 患者血小板活化率及血小板中 IL-1β的表达率并探讨其意义。方法用流式细胞术检测50例 RA 患者（其中22例严重活动、28例中低活动）和30名健康对照者外周血血小板活化率及血小板中 IL-1β的表达水平，检测炎症活动指标 ESR、CRP，评价患者关节活动性（DAS28评分）。采用 t 检验和直线相关分析进行统计学分析。结果 RA 组血小板活化率（19.2±4.8）较对照组（9.0±2.9）升高（t=10.5，P=0.001），血小板中 IL-1β的表达率（41±11）较对照组（21±10）升高（t=9.01，P=0.000）；RA 严重活动组血小板活化率（22±4）较中低活动组（17±4）升高（t=3.96，P=0.001），血小板中 IL-1β的表达率（45±10）较中低活动组（38±10）升高（t=2.329，P=0.024）；IL-1β的表达与 ESR、CRP 和 DAS28评分呈正相关（r 值和 P 值分别是0.576，0.578，0.618和0.000，0.000，0.000）。结论 RA 患者血小板异常活化并合成IL-1β，与 RA 病情活动显著相关，可作为 RA 炎症活动的监测指标。血小板可能通过合成分泌 IL-1β在RA 中发挥致炎作用。
목적：검측 RA 환자혈소판활화솔급혈소판중 IL-1β적표체솔병탐토기의의。방법용류식세포술검측50례 RA 환자（기중22례엄중활동、28례중저활동）화30명건강대조자외주혈혈소판활화솔급혈소판중 IL-1β적표체수평，검측염증활동지표 ESR、CRP，평개환자관절활동성（DAS28평분）。채용 t 검험화직선상관분석진행통계학분석。결과 RA 조혈소판활화솔（19.2±4.8）교대조조（9.0±2.9）승고（t=10.5，P=0.001），혈소판중 IL-1β적표체솔（41±11）교대조조（21±10）승고（t=9.01，P=0.000）；RA 엄중활동조혈소판활화솔（22±4）교중저활동조（17±4）승고（t=3.96，P=0.001），혈소판중 IL-1β적표체솔（45±10）교중저활동조（38±10）승고（t=2.329，P=0.024）；IL-1β적표체여 ESR、CRP 화 DAS28평분정정상관（r 치화 P 치분별시0.576，0.578，0.618화0.000，0.000，0.000）。결론 RA 환자혈소판이상활화병합성IL-1β，여 RA 병정활동현저상관，가작위 RA 염증활동적감측지표。혈소판가능통과합성분비 IL-1β재RA 중발휘치염작용。
Objective To evaluate the significance of platelet activation and the expression of inter-leukin (IL)-1β in patients with RA. Methods The activation of platelets and the expression of IL-1β in pla-telets in 50 RA patients(22 high-active, 28 mediate/low active ) and 30 normal controls were determined us-ing flow cytometry. Meanwhile, inflammatory indicators such as erythrocyte sedimentation(ESR), C-reactive protein (CRP) and DAS28 were also recorded. T test and correlation analysis were performed. Results The platelet activation in RA group(19.2±4.8) was higher than the control group(9.0±2.9)(t=10.5, P=0.001). The expression of IL-1β in platelets in RA group(41±11) was higher than control group(21±8)(t=9.01, P =0.000) .The platelet activation in high-active RA group(22 ±4) was higher than mediate/low active RA group(17 ±4)(t =3.96,P =0.001). The expression of IL-1β in platelets in high-active RA group(45 ±10) was higher than mediate/low active RA group (38 ±10)(t =2.329,P =0.024). The expression of IL-1β in platelets in RA group was positively correlated with the level of ESR、CRP and DAS28 (r value and P value were 0.576, 0.578, 0.618 and 0.000, 0.000, 0.000 respectively). Conclusion The platelets of patients with RA are activated and may suggest that IL-1β, which may associate with disease activity. Our research suggest that platelet may play a role in the inflammatory process of RA by secreting IL-1β.