中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2014年
10期
661-664
,共4页
林丽%叶玲英%殷健%张立斌%徐沪济
林麗%葉玲英%慇健%張立斌%徐滬濟
림려%협령영%은건%장립빈%서호제
Th17 细胞%白细胞介素 17%肿瘤坏死因子 α%关节炎, 类风湿%脊柱炎, 强直性
Th17 細胞%白細胞介素 17%腫瘤壞死因子 α%關節炎, 類風濕%脊柱炎, 彊直性
Th17 세포%백세포개소 17%종류배사인자 α%관절염, 류풍습%척주염, 강직성
Th17 cells%Interleukin-17%Tumor necrosis factor-alpha%Arthritis,rheumatoid%Spondylitis,ankylosing
目的初步探讨外周血 Th17细胞亚群的比例以及 IL-17的表达水平在 RA 和 AS 患者接受 TNF-α拮抗剂治疗前后的改变及其意义。方法选择 RA 患者27例和 AS 患者22例,2种疾病中各有14例患者接受 TNF-α拮抗剂治疗40周。对照组24名来源于健康献血者。采用流式细胞术检测外周血 CD4+T 细胞中 Th17细胞亚群的比例,ELISA 检测外周血 IL-17表达水平。符合正态分布数据采用2个独立样本 t 检验,不符合正态分布则采用 Wilcoxon 秩和检验,患者治疗前后的比较采用配对 t 检验。结果治疗前,RA 和 AS 患者外周血 CD4+ T 细胞中 Th17细胞亚群的比例显著高于健康对照组[RA 1.03%(0.66%,1.78%)与健康对照0.50%(0.43%,0.67%),Z=-3.236,P<0.01;AS(1.16±0.09)%与健康对照(0.59±0.06)%,t=5.226,P<0.01]。同样,IL-17的表达水平在2组疾病中也显著升高[RA(32.3±2.5) pg/ml,健康对照(14.3±2.5) pg/ml,t=5.070,P<0.01;AS 28.98(23.84,36.14) pg/ml,健康对照11.84(5.33,22.12) pg/ml,Z=-4.103,P<0.01]。 TNF-α拮抗剂治疗后,2组疾病 CD4+T 细胞中 Th17细胞亚群比例无明显变化[RA 驻(0.1045±0.2126)%;AS 驻(0.0025±0.1838)%],但 IL-17表达水平则明显下降[RA 驻(-13.5±5.0) pg/ml;AS 驻(-16.0±1.9) pg/ml]。结论 Th17细胞及其分泌的细胞因子 IL-17在 RA 和 AS 的发病机制中起重要作用,TNF-α拮抗剂对 AS 和 RA 患者 Th17细胞亚群炎症细胞因子的分泌功能有明显的抑制作用,但40周的治疗仍不能降低 Th17细胞比例,这可能是 TNF-α拮抗剂短期治疗后疾病复发的原因之一。
目的初步探討外週血 Th17細胞亞群的比例以及 IL-17的錶達水平在 RA 和 AS 患者接受 TNF-α拮抗劑治療前後的改變及其意義。方法選擇 RA 患者27例和 AS 患者22例,2種疾病中各有14例患者接受 TNF-α拮抗劑治療40週。對照組24名來源于健康獻血者。採用流式細胞術檢測外週血 CD4+T 細胞中 Th17細胞亞群的比例,ELISA 檢測外週血 IL-17錶達水平。符閤正態分佈數據採用2箇獨立樣本 t 檢驗,不符閤正態分佈則採用 Wilcoxon 秩和檢驗,患者治療前後的比較採用配對 t 檢驗。結果治療前,RA 和 AS 患者外週血 CD4+ T 細胞中 Th17細胞亞群的比例顯著高于健康對照組[RA 1.03%(0.66%,1.78%)與健康對照0.50%(0.43%,0.67%),Z=-3.236,P<0.01;AS(1.16±0.09)%與健康對照(0.59±0.06)%,t=5.226,P<0.01]。同樣,IL-17的錶達水平在2組疾病中也顯著升高[RA(32.3±2.5) pg/ml,健康對照(14.3±2.5) pg/ml,t=5.070,P<0.01;AS 28.98(23.84,36.14) pg/ml,健康對照11.84(5.33,22.12) pg/ml,Z=-4.103,P<0.01]。 TNF-α拮抗劑治療後,2組疾病 CD4+T 細胞中 Th17細胞亞群比例無明顯變化[RA 駐(0.1045±0.2126)%;AS 駐(0.0025±0.1838)%],但 IL-17錶達水平則明顯下降[RA 駐(-13.5±5.0) pg/ml;AS 駐(-16.0±1.9) pg/ml]。結論 Th17細胞及其分泌的細胞因子 IL-17在 RA 和 AS 的髮病機製中起重要作用,TNF-α拮抗劑對 AS 和 RA 患者 Th17細胞亞群炎癥細胞因子的分泌功能有明顯的抑製作用,但40週的治療仍不能降低 Th17細胞比例,這可能是 TNF-α拮抗劑短期治療後疾病複髮的原因之一。
목적초보탐토외주혈 Th17세포아군적비례이급 IL-17적표체수평재 RA 화 AS 환자접수 TNF-α길항제치료전후적개변급기의의。방법선택 RA 환자27례화 AS 환자22례,2충질병중각유14례환자접수 TNF-α길항제치료40주。대조조24명래원우건강헌혈자。채용류식세포술검측외주혈 CD4+T 세포중 Th17세포아군적비례,ELISA 검측외주혈 IL-17표체수평。부합정태분포수거채용2개독립양본 t 검험,불부합정태분포칙채용 Wilcoxon 질화검험,환자치료전후적비교채용배대 t 검험。결과치료전,RA 화 AS 환자외주혈 CD4+ T 세포중 Th17세포아군적비례현저고우건강대조조[RA 1.03%(0.66%,1.78%)여건강대조0.50%(0.43%,0.67%),Z=-3.236,P<0.01;AS(1.16±0.09)%여건강대조(0.59±0.06)%,t=5.226,P<0.01]。동양,IL-17적표체수평재2조질병중야현저승고[RA(32.3±2.5) pg/ml,건강대조(14.3±2.5) pg/ml,t=5.070,P<0.01;AS 28.98(23.84,36.14) pg/ml,건강대조11.84(5.33,22.12) pg/ml,Z=-4.103,P<0.01]。 TNF-α길항제치료후,2조질병 CD4+T 세포중 Th17세포아군비례무명현변화[RA 주(0.1045±0.2126)%;AS 주(0.0025±0.1838)%],단 IL-17표체수평칙명현하강[RA 주(-13.5±5.0) pg/ml;AS 주(-16.0±1.9) pg/ml]。결론 Th17세포급기분비적세포인자 IL-17재 RA 화 AS 적발병궤제중기중요작용,TNF-α길항제대 AS 화 RA 환자 Th17세포아군염증세포인자적분비공능유명현적억제작용,단40주적치료잉불능강저 Th17세포비례,저가능시 TNF-α길항제단기치료후질병복발적원인지일。
Objective To explore the effect of tumor necrosis factor-alpha(TNF-α) blockade therapy on circulating Th17 cell percentage and serum interleukin (IL)-17 level in patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS). Methods Twenty-seven RA and 22 AS patients were recruited, of which 14 cases from both diseases received 40 weeks TNF blockade therapy. Twenty-four healthy blood donors were used as controls. The frequencies of circulating Th17 cells were determined by flowcytometry, and serum IL-17 level were measured by enzyme linked immunosorbent assay(ELISA). Results Significantly higher baseline circulating Th17 cells were observed in active RA and AS patients compared with the healthy controls[RA 1.03%(0.66%,1.78%) vs controls 0.50%(0.43%,0.67%), Z=-3.236, P<0.01; AS(1.16±0.09)%vs controls (0.59 ±0.061)% , t =5.226, P <0.01]. Similarly, serum IL-17 level were significantly elevated in patients with both diseases compared with controls[RA(32.3±2.5) pg/ml vs controls(14.3±2.5) pg/ml, t=5.070, P<0.01; AS 28.98(23.84,36.14) pg/ml vs controls 11.84(5.33,22.12) pg/ml, Z=-4.103, P<0.01]. After TNF-α blockade therapy, serum IL-17 was significantly decreased in both diseases groups[RA △(-13.5± 5.0) pg/ml and AS △(-16.0±1.9) pg/ml]. In contrast, no significant differences were found in the frequencies of circulating Th17 cells[RA △(0.104 5±0.212 6)% and AS △(0.002 5±0.183 8)%]. Conclusion Th17 cells and IL-17 have been implicated in the pathogenesis of RA and AS. TNF-α blockade can partially inhibit the function of Th17 cells. However, it is unable to reduce the frequencies of these cells in the circulation after 40 weeks therapy, which may explain the reasons for the relapse.