CAJ | 학술논문

提取绵羊输卵管上皮细胞总RNA，采用Primer 5.0软件设计引物，运用RT-PCR技术扩增Ghrelin基因。结果显示，经测序和序列比对鉴定，该方法成功地扩增到了大小为200 bp的目的基因，表明RT-PCR技术是检测绵羊输卵管上皮细胞中Ghrelin基因的有效手段，可为今后研究不同激素水平下绵羊输卵管组织中Ghrelin基因的表达量变化及进一步体外试验奠定基础。
제취면양수란관상피세포총RNA，채용Primer 5.0연건설계인물，운용RT-PCR기술확증Ghrelin기인。결과현시，경측서화서렬비대감정，해방법성공지확증도료대소위200 bp적목적기인，표명RT-PCR기술시검측면양수란관상피세포중Ghrelin기인적유효수단，가위금후연구불동격소수평하면양수란관조직중Ghrelin기인적표체량변화급진일보체외시험전정기출。
Total RNA was extracted from oviduct epithelial cells of sheep,primers were designed by Primer 5.0 software,and Ghrelin gene was amplified by RT-PCR. The results showed that 200 bp of aim gene was obtained successfully by RT-PCR, which indicated that RT-PCR technique was an efficient mean to detect expression of Ghrelin gene in oviduct epithelial cells of sheep. The results lay the basis for studying expression change of Ghrelin gene in oviduct tissues of sheep under different hormone levels and further in vitro test.