CAJ | 학술논문

目的：观察脑缺血再灌注损伤后SD大鼠不同时间点神经功能评分及梗死体积的变化，探讨脑心通对它们的影响。方法线栓法制作大鼠大脑中动脉缺血（Middle cerebral artery occlusion，MCAO）模型，选用健康雄性SD大鼠68只，随机分成四组：假手术组；MCAO模型组；MCAO+脑心通胶囊低剂量组；MCAO+脑心通胶囊高剂量组。每组再按照脑缺血再灌注后第3d、第7d、第14d和第21d 4个不同时间点进行神经功能评分及2，3，5-氯化三苯基四氮唑（2，3，5-Triphenyltetrazolium chloride，TTC）染色测定大鼠脑缺血再灌注后梗死体积。结果除假手术组外，其余三组实验鼠在缺血再灌注后4个不同时间点之间比较神经功能评分具有差异（P<0.01）；不同剂量脑心通组与MCAO模型组比较差异有统计学意义（P<0.05）；但脑心通高、低剂量组之间比较无明显差异（P>0.05）。 TTC染色发现缺血再灌注后第7d各组大鼠脑梗死体积最大；不同时间点之间比较差异有统计学意义（P<0.05）；不同剂量的脑心通实验组与模型组之间比较，差异具有统计学意义（P<0.01）。结论脑心通胶囊对改善缺血再灌注损伤后实验鼠神经功能评分和减轻梗死体积有促进作用，但对神经功能评分的影响与药物剂量不相关。
목적：관찰뇌결혈재관주손상후SD대서불동시간점신경공능평분급경사체적적변화，탐토뇌심통대타문적영향。방법선전법제작대서대뇌중동맥결혈（Middle cerebral artery occlusion，MCAO）모형，선용건강웅성SD대서68지，수궤분성사조：가수술조；MCAO모형조；MCAO+뇌심통효낭저제량조；MCAO+뇌심통효낭고제량조。매조재안조뇌결혈재관주후제3d、제7d、제14d화제21d 4개불동시간점진행신경공능평분급2，3，5-록화삼분기사담서（2，3，5-Triphenyltetrazolium chloride，TTC）염색측정대서뇌결혈재관주후경사체적。결과제가수술조외，기여삼조실험서재결혈재관주후4개불동시간점지간비교신경공능평분구유차이（P<0.01）；불동제량뇌심통조여MCAO모형조비교차이유통계학의의（P<0.05）；단뇌심통고、저제량조지간비교무명현차이（P>0.05）。 TTC염색발현결혈재관주후제7d각조대서뇌경사체적최대；불동시간점지간비교차이유통계학의의（P<0.05）；불동제량적뇌심통실험조여모형조지간비교，차이구유통계학의의（P<0.01）。결론뇌심통효낭대개선결혈재관주손상후실험서신경공능평분화감경경사체적유촉진작용，단대신경공능평분적영향여약물제량불상관。
Objective To observe the changes of Neurological function scores and infarct volumes at different time after cere-bral ischemia-reperfusion injury in rats. And to explore the effects of Naoxintong treatment. Methods Middle cerebral artery oc-clusion (MCAO) model was constructed with suture method. 68 healthy male SD rats were enrolled in this study. These rats were randomly divided into four groups: pseudo-surgery group,MACO group,MACO+low dose of Naoxintong group,MACO+ high dose of Naoxintong group. Neurological function scores and infarct volume were assessed on 3th,7th,14th and 21th day after cerebral ischemia and reperfusion by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Results The three experiment groups all showed vary degrees of neurological function deficit except for pseudo-surgery group. Neurological function scores were decreased along with time,further neurological function scores at different time were significant in experiment groups (P<0.01). There is significant difference between different doses Naoxintong groups and MACO group (P<0.05). While high and low dose of Naoxintong group showed no significant difference (P>0.05). Rat infarct volumes assessed with 2,3,5-triphenyltetrazolium chloride (TTC) in different experiment group were both highest on 7th day after ischemia reperfusion by TTC staining ,and there were different significance at different time (P<0.05). The infarct volumes were statistically significant difference among high dose Naoxintong ,low dose Naoxin-tong,and MACO model (P<0.01). Conclusion Naoxintong could improve neurological function scores and reduce the infract vol-umes,while the effect on neurological function dose not correlate with dose of Naoxintong after ischemic reperfusion.