中国民族民间医药
中國民族民間醫藥
중국민족민간의약
CHINESE JOURNAL OF ETHNOMEDICINE AND ETHNOPHARMACY
2014年
21期
12-14
,共3页
刘晓鹏%姜宁%丁峰峻%丁振国%李力%梁玉堂
劉曉鵬%薑寧%丁峰峻%丁振國%李力%樑玉堂
류효붕%강저%정봉준%정진국%리력%량옥당
连钱草%总黄酮%响应面法%提取工艺%优化
連錢草%總黃酮%響應麵法%提取工藝%優化
련전초%총황동%향응면법%제취공예%우화
Glechoma longituba%total flavonoids%response surface methodology%extraction process%optimization
目的:优化连钱草总黄酮提取工艺。方法:以连钱草总黄酮提取的四个主要因素(提取时间、提取温度、液固比、乙醇)为自变量,黄酮提取率为响应值,采用响应面法Box-Behnken试验建立回归方程,优化连钱草总黄酮的提取工艺。结果:经过验证,当提取时间1.5h,提取温度41℃,液固比23∶1,乙醇浓度90%时,黄酮提取率可达6.32%~6.47%。结论:本研究优化出了连钱草总黄酮的提取工艺,该工艺能很好地指导实践生产,为连钱草总黄酮的进一步开发提供了理论基础。
目的:優化連錢草總黃酮提取工藝。方法:以連錢草總黃酮提取的四箇主要因素(提取時間、提取溫度、液固比、乙醇)為自變量,黃酮提取率為響應值,採用響應麵法Box-Behnken試驗建立迴歸方程,優化連錢草總黃酮的提取工藝。結果:經過驗證,噹提取時間1.5h,提取溫度41℃,液固比23∶1,乙醇濃度90%時,黃酮提取率可達6.32%~6.47%。結論:本研究優化齣瞭連錢草總黃酮的提取工藝,該工藝能很好地指導實踐生產,為連錢草總黃酮的進一步開髮提供瞭理論基礎。
목적:우화련전초총황동제취공예。방법:이련전초총황동제취적사개주요인소(제취시간、제취온도、액고비、을순)위자변량,황동제취솔위향응치,채용향응면법Box-Behnken시험건립회귀방정,우화련전초총황동적제취공예。결과:경과험증,당제취시간1.5h,제취온도41℃,액고비23∶1,을순농도90%시,황동제취솔가체6.32%~6.47%。결론:본연구우화출료련전초총황동적제취공예,해공예능흔호지지도실천생산,위련전초총황동적진일보개발제공료이론기출。
Objective To optimize the extraction process of total flavonoids from Glechoma longituba. Methods The regression e-quation was established using response surface methodology with Box-Behnken test by four main factors including extraction time,ex-traction temperature,liquid -solid ratio and ethanol concentration as independent variables and yield of total flavonoids as re-sponse.Results The yield of total flavonoids from Glechoma longituba reached 6.32%~6.47%when the validated process was extrac-tion time 1.5h,extraction temperature 41℃,liquid " solid ratio 23∶1 and ethanol concentration 90%. Conclusion The optimized process is obtained and could be a good production guide. The present study provides a theoretical basis for the further development of total flavonoids from Glechoma longituba.