中国民族民间医药
中國民族民間醫藥
중국민족민간의약
CHINESE JOURNAL OF ETHNOMEDICINE AND ETHNOPHARMACY
2014年
21期
8-9
,共2页
梁霄%莫建光%刘布鸣%黄艳%高微%黎凯群%黎奕良
樑霄%莫建光%劉佈鳴%黃豔%高微%黎凱群%黎奕良
량소%막건광%류포명%황염%고미%려개군%려혁량
金花茶叶%齐墩果酸%芦丁%人参皂苷Rg1%TLC
金花茶葉%齊墩果痠%蘆丁%人參皂苷Rg1%TLC
금화다협%제돈과산%호정%인삼조감Rg1%TLC
Camellia%oleanolic acid%rutin%ginsenosides Rg1%TLC
目的：建立金花茶叶的薄层色谱鉴别方法。方法：采用薄层色谱法分别鉴别金花茶叶中的齐墩果酸、芦丁和人参皂苷Rg1等化学成分。结果：齐墩果酸、芦丁和人参皂苷Rg1分别在以石油醚-乙酸乙酯-丙酮（7∶2∶1）、乙酸乙酯-甲醇-水-甲酸（9∶2∶1∶0.1）和氯仿-乙酸乙酯-甲醇-水（3∶8∶4.4∶1.6）为展开剂的条件下获得理想分离，且在同一点样量和展开条件下，三个金花茶品种中的齐墩果酸、芦丁、人参皂苷Rg1斑点均有明显差异。结论：该方法简便、快速，可更好地控制金花茶叶的药材质量。
目的：建立金花茶葉的薄層色譜鑒彆方法。方法：採用薄層色譜法分彆鑒彆金花茶葉中的齊墩果痠、蘆丁和人參皂苷Rg1等化學成分。結果：齊墩果痠、蘆丁和人參皂苷Rg1分彆在以石油醚-乙痠乙酯-丙酮（7∶2∶1）、乙痠乙酯-甲醇-水-甲痠（9∶2∶1∶0.1）和氯倣-乙痠乙酯-甲醇-水（3∶8∶4.4∶1.6）為展開劑的條件下穫得理想分離，且在同一點樣量和展開條件下，三箇金花茶品種中的齊墩果痠、蘆丁、人參皂苷Rg1斑點均有明顯差異。結論：該方法簡便、快速，可更好地控製金花茶葉的藥材質量。
목적：건립금화다협적박층색보감별방법。방법：채용박층색보법분별감별금화다협중적제돈과산、호정화인삼조감Rg1등화학성분。결과：제돈과산、호정화인삼조감Rg1분별재이석유미-을산을지-병동（7∶2∶1）、을산을지-갑순-수-갑산（9∶2∶1∶0.1）화록방-을산을지-갑순-수（3∶8∶4.4∶1.6）위전개제적조건하획득이상분리，차재동일점양량화전개조건하，삼개금화다품충중적제돈과산、호정、인삼조감Rg1반점균유명현차이。결론：해방법간편、쾌속，가경호지공제금화다협적약재질량。
Objective To establish a method for identification of Camellia by Thin-Layer Chromatography (TLC).Methods The specific compouds,oleanolic acid,rutin and ginsenosides Rg1 were studied by TLC in three different Camellia species.Results These three kinds of ingredients were all separated very well by the Ligroin-acetoacetate -acetone (7∶2∶1 ),Acetoacetate -methanol-aquaefer-formic acid (9∶2∶1∶0.1 ),Chloroform-acetoacetate-methanol-aquaefer (3∶8∶4.4∶1.6 ),and under the same condi-tions,the spots of oleanolic acid,rutin and ginsenosides Rg1 were significantly in different three varieties of Camellia.Conclusion This method was simple ,quick,and can better control the quality of the Camellia.
