CAJ | 학술논문

目的：观察鼻咽癌患者血清中细胞角蛋白片段CK19-2G2、CyFra21-1浓度及E月病毒抗体灾悦A-IgA、EA-IgA滴度水平，评价以上四项肿瘤标志物对鼻咽癌诊断和预后的应用价值。方法分别检测72例正常健康人和47例放疗前后的未分化型非角化性鼻咽癌患者血清CK19-2G2、悦赠云则葬21-1浓度和灾悦A-IgA/EA-IgA滴度，CK19-2G2应用北京同生时代公司的仪器及试剂盒(化学发光法)检测；悦赠云则葬21-1应用罗氏电化学发光法检测；灾悦A-IgA应用免疫酶法检测。结果未分化型非角化性鼻咽癌患者血清CK19-2G2、悦赠云则葬21-1和灾悦A-IgA/EA-IgA的阳性率分别为：32.69%、46.15%、98.08%和71.15%，健康人群以上四项指标的阳性率分别为：9.32%、9.72%、16.67%和0， P<0.01；与病理诊断的一致性检验显示以上四项指标的运葬责责葬值分别为：0.355、0.418、0.78和0.76；四项指标与临床分期有统计学意义(χ2分别为：69.04、28.21、90.36和70.49， P <0.01)；未分化型鼻咽鳞癌患者放疗前后CK19-2G2和悦赠云则葬21-1阳性率呈下降趋势。结论血清CK19-2G2、悦赠云则葬21-1水平在鼻咽鳞癌患者和健康人群中有显著性差异，E月灾灾悦A-IgA/EA-IgA滴度检测对鼻咽癌的诊断有较高准确率；鼻咽鳞癌患者血清CK19-2G2、悦赠云则葬21-1水平与病理分期相关；血清CK19-2G2、悦赠云则葬21-1的水平可能对鼻咽鳞癌患者的预后评价有一定的价值。
목적：관찰비인암환자혈청중세포각단백편단CK19-2G2、CyFra21-1농도급E월병독항체재열A-IgA、EA-IgA적도수평，평개이상사항종류표지물대비인암진단화예후적응용개치。방법분별검측72례정상건강인화47례방료전후적미분화형비각화성비인암환자혈청CK19-2G2、열증운칙장21-1농도화재열A-IgA/EA-IgA적도，CK19-2G2응용북경동생시대공사적의기급시제합(화학발광법)검측；열증운칙장21-1응용라씨전화학발광법검측；재열A-IgA응용면역매법검측。결과미분화형비각화성비인암환자혈청CK19-2G2、열증운칙장21-1화재열A-IgA/EA-IgA적양성솔분별위：32.69%、46.15%、98.08%화71.15%，건강인군이상사항지표적양성솔분별위：9.32%、9.72%、16.67%화0， P<0.01；여병리진단적일치성검험현시이상사항지표적운장책책장치분별위：0.355、0.418、0.78화0.76；사항지표여림상분기유통계학의의(χ2분별위：69.04、28.21、90.36화70.49， P <0.01)；미분화형비인린암환자방료전후CK19-2G2화열증운칙장21-1양성솔정하강추세。결론혈청CK19-2G2、열증운칙장21-1수평재비인린암환자화건강인군중유현저성차이，E월재재열A-IgA/EA-IgA적도검측대비인암적진단유교고준학솔；비인린암환자혈청CK19-2G2、열증운칙장21-1수평여병리분기상관；혈청CK19-2G2、열증운칙장21-1적수평가능대비인린암환자적예후평개유일정적개치。
Objective To evaluate the diagnostic and prognostic utility of serum cytokeratin fragment:CK19-2G2, CyFra21-1 level and EBV VCA-IgA, EA-IgA titers in patients with nasopharyngeal squamous cell carcinoma. Methods 72 cases of normal volunteers and 47 cases of patients with undifferentiated nasopharyngeal squamous cell carcinoma before radiotherapy (pre-RT) were detected by serum CK19-2G2 (Tongsheng Shidai, Beijing), CyFra21-1 (Roche diagnosis) level and EBV VCA-IgA/EA-IgA titers (immunoenzyme technique). Results The positive rate of CK19-2G2, CyFra21-1 and EBV VCA-IgA/EA-IgA in undifferentiated nasopharyngeal squamous cell carcinoma patients were:32.69%, 46.15%, 98.08%and 71.15%, respectively;The positive rate of the above four tests in normal volunteers was: 9.32%, 9.72%, 16.67% and 0, respectively, P <0.01. The correlation of the four tests and pathological staging were statistically analyzed, Kappa value was: 0.355, 0.418, 0.78 and 0.76, χ2: 69.04, 28.21, 90.36 and 70.49, respectively, P <0.01. The positive rate of undifferentiated nasopharyngeal squamous cell carcinoma patients reduced after radiotherapy (post-RT), followed by the mass reduced. Conclusions Serum CK19-2G2 and CyFra21-1 level have significant difference between undifferentiated nasopharyngeal squamous cell carcinoma patients and the normal control. EBV VCA-IgA/EA-IgA titer detection have higher accuracy in serological diagnosis of nasopharyngeal carcinoma. Serum CK19-2G2 and CyFra21-1 level will be the valuable prognostic prediction factors of undifferentiated nasopharyngeal squamous cell carcinoma.