CAJ | 학술논문

目的：临床上建立一种快速、准确测定肿瘤患儿血浆中甲氨蝶呤浓度方法。方法取肿瘤患儿血浆250μ蕴，加入7豫高氯酸200μ蕴，涡旋1皂蚤灶，室温高速离心5皂蚤灶(15000×早)，取上清液5μ蕴进样分析。色谱条件：色谱柱为月耘匀悦18柱(50皂皂×2.1皂皂，1.7μ皂)；流速为0.3皂蕴·皂蚤灶-1；流动相为乙腈-40皂燥造·蕴-1磷酸二氢钾缓冲液(91：9，责匀越4.5)；柱温为30℃；进样量：5μ蕴；检测波长为304灶皂。结果血浆中M栽载色谱峰分离良好，无干扰，曲线为赠越41729曾-810.29，砸2越0.9997，回收率在90豫~110豫之间。结论哉孕蕴悦操作简便，适用于血浆中M栽载快速监测。
목적：림상상건립일충쾌속、준학측정종류환인혈장중갑안접령농도방법。방법취종류환인혈장250μ온，가입7예고록산200μ온，와선1조조조，실온고속리심5조조조(15000×조)，취상청액5μ온진양분석。색보조건：색보주위월운균열18주(50조조×2.1조조，1.7μ조)；류속위0.3조온·조조조-1；류동상위을정-40조조조·온-1린산이경갑완충액(91：9，책균월4.5)；주온위30℃；진양량：5μ온；검측파장위304조조。결과혈장중M재재색보봉분리량호，무간우，곡선위증월41729증-810.29，잡2월0.9997，회수솔재90예~110예지간。결론재잉온열조작간편，괄용우혈장중M재재쾌속감측。
Objective To establish a rapid and accurate method to determine the plasma concentration of methotrexate in children with tumor. Methods 0.25μL plasma specimen taken from tumorous children was extracted with 7%perchloric acid and swirled 1 min, then it was high-speed centrifuged 5 min (15 000íg). 5μL supernate was injected in UPLC assay for analysis. The analytical column was BEH C18 (50 mmí2.1 mm, 1.7μm), the flow rate was 0.3 mL·min-1, and the mobile phase was the buffer of acetonitrile-40 mol·L-1 and NaH2PO4 (91: 9, pH = 4.5). The column temperature was 30 ℃, the sample size was 5 μL, and the wavelength was 304 nm. Results The chromatographic peak of methotrexate in plasma separated good without interference. The curve was y=41 729x-810.29, R2=0.9 997, the recycle rate was between 90%and 100%. Conclusions UPLC is easy to operate and fit for fast determination of MTX in human plasma.