CAJ | 학술논문

目的：探讨玻璃化冷冻对大鼠卵泡中线粒体的形态和功能的影响。方法采用雌性Sprague-Dawley大鼠分离后的卵泡作为研究对象，分为I组(新鲜组)和II组(玻璃化冷冻组)，分别用实时荧光定量PCR检测卵泡中线粒体DNA拷贝数，用荧光显微镜观察线粒体分布情况，用生物发光实验检测卵泡中ATP含量。结果 I组的线粒体DNA拷贝数为638000±65101，明显高于II组的线粒体DNA拷贝数220000±58474，差异具有统计学意义(P<0.05)。 I组中的线粒体有典型的丝状、皱褶分布等特点，而II组中的线粒体则分散存在，失去了正常的分布情况。 II组卵泡的ATP含量明显低于I组的，差异有统计学意义(P<0.05)。结论玻璃化冷冻过程会对卵泡线粒体的形态、分布和产生线粒体的功能造成不同程度的损伤，从而可能会影响卵泡的生长发育。
목적：탐토파리화냉동대대서란포중선립체적형태화공능적영향。방법채용자성Sprague-Dawley대서분리후적란포작위연구대상，분위I조(신선조)화II조(파리화냉동조)，분별용실시형광정량PCR검측란포중선립체DNA고패수，용형광현미경관찰선립체분포정황，용생물발광실험검측란포중ATP함량。결과 I조적선립체DNA고패수위638000±65101，명현고우II조적선립체DNA고패수220000±58474，차이구유통계학의의(P<0.05)。 I조중적선립체유전형적사상、추습분포등특점，이II조중적선립체칙분산존재，실거료정상적분포정황。 II조란포적ATP함량명현저우I조적，차이유통계학의의(P<0.05)。결론파리화냉동과정회대란포선립체적형태、분포화산생선립체적공능조성불동정도적손상，종이가능회영향란포적생장발육。
Objective To investigate the effect of vitrified cryopreservation on morphology and function of mitochondria in rats' follicles. Methods The isolated follicles of female Sprague-Dawley rats were used as experimental animal model and divided into group I (fresh follicles) and group II (vitrified cryopreservation). The copy number of mitochondria DNA (mtDNA) was detected by real time PCR, the distribution of mitochondria was examined by fluorescent microscope, the ATP content of follicles was detected by bioluminescence assay. Results The copy number of mitochondria DNA of group I was 638 000±65 101, significantly higher than 220 000±58 474 of group II, with statistically significant difference (P <0.05). The distribution of mitochondria of group I was typical threadiness, while it was disperse in group II. The ATP content of follicles of group II was significantly lower than that of group I, with statistically significant difference (P <0.05). Conclusions Vitrified cryopreservation damage the morphology, distribution and function of producing mitochondria of follicular mitochondria, thus may be affect the growth and development of follicles.