CAJ | 학술논문

为了获得1株高效转化木糖为木糖醇的酵母菌株，利用从土壤中筛选的酵母菌株转化玉米芯水解液生产木糖醇，经HPLC测得木糖醇产量，对筛选的具有产木糖醇能力的酵母菌株DQ11进行形态学的初步鉴定及26S rDNA序列分析，并以序列同源性为基础构建相关属种的系统发育树。结果表明，该菌株发酵48 h后木糖醇产量为3.34 g/L。同源性分析结果表明，分离株DQ11与季也蒙毕赤酵母菌处于同一进化分支中，相似性达99%以上，因此将筛选的高产木糖醇分离株DQ11鉴定为季也蒙毕赤酵母菌。
위료획득1주고효전화목당위목당순적효모균주，이용종토양중사선적효모균주전화옥미심수해액생산목당순，경HPLC측득목당순산량，대사선적구유산목당순능력적효모균주DQ11진행형태학적초보감정급26S rDNA서렬분석，병이서렬동원성위기출구건상관속충적계통발육수。결과표명，해균주발효48 h후목당순산량위3.34 g/L。동원성분석결과표명，분리주DQ11여계야몽필적효모균처우동일진화분지중，상사성체99%이상，인차장사선적고산목당순분리주DQ11감정위계야몽필적효모균。
In order to obtain a yeast strain that can highly produce xylitol, xylitol from corn cob hydrolysate which is transformed by yeast strain screened from the soil is produced. It is measured that the yield of xylitol by HPLC, and made initial morphologic and 26S rDNA sequence analyses of screened yeast strain DQ11 that can produce xylitol, and built phylogenetic tree of correlative generic species on the basis of sequence homology. The result shows this yeast strain produces 3.34 g/L of xyolitol after 48 hours' fermentation. The result of homology analysis shows that the isolated strain DQ11 is in the same evolutionary branch with Pichia guilliermondii with a similarity of over 99%. So DQ11 is identified as Pichia guilliermondii.