中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
11期
1977-1979,1980
,共4页
化妆品%防晒剂%高效液相色谱法
化妝品%防曬劑%高效液相色譜法
화장품%방쇄제%고효액상색보법
Cosmetics%Sun-screening agents%HPLC
目的：改进和优化《化妆品卫生规范》2007年中15种防晒剂的HPLC测定法,以便更好地对化妆品中限用的化学防晒剂进行质量监控。方法：采用经改进的混合液提取样品,以 Accurasil C18(250 mm ×4.6 mm,5μm)为分析色谱柱,甲醇-四氢呋喃-水(冰醋酸调pH至2.60)为流动相梯度洗脱,流速：1.0 ml·min-1,柱温：25℃,检测波长：311 nm。结果：15种防晒剂的线性良好,在0.85%~1.56%之间,r均>0.9995,检出限为1.9~18.7 ng,方法回收率为97.5%~113.7%,RSD(n=9)。结论：改进后的方法结果可靠,对《化妆品卫生规范》2007版防晒剂测定方法是有效的优化和补充。
目的：改進和優化《化妝品衛生規範》2007年中15種防曬劑的HPLC測定法,以便更好地對化妝品中限用的化學防曬劑進行質量鑑控。方法：採用經改進的混閤液提取樣品,以 Accurasil C18(250 mm ×4.6 mm,5μm)為分析色譜柱,甲醇-四氫呋喃-水(冰醋痠調pH至2.60)為流動相梯度洗脫,流速：1.0 ml·min-1,柱溫：25℃,檢測波長：311 nm。結果：15種防曬劑的線性良好,在0.85%~1.56%之間,r均>0.9995,檢齣限為1.9~18.7 ng,方法迴收率為97.5%~113.7%,RSD(n=9)。結論：改進後的方法結果可靠,對《化妝品衛生規範》2007版防曬劑測定方法是有效的優化和補充。
목적：개진화우화《화장품위생규범》2007년중15충방쇄제적HPLC측정법,이편경호지대화장품중한용적화학방쇄제진행질량감공。방법：채용경개진적혼합액제취양품,이 Accurasil C18(250 mm ×4.6 mm,5μm)위분석색보주,갑순-사경부남-수(빙작산조pH지2.60)위류동상제도세탈,류속：1.0 ml·min-1,주온：25℃,검측파장：311 nm。결과：15충방쇄제적선성량호,재0.85%~1.56%지간,r균>0.9995,검출한위1.9~18.7 ng,방법회수솔위97.5%~113.7%,RSD(n=9)。결론：개진후적방법결과가고,대《화장품위생규범》2007판방쇄제측정방법시유효적우화화보충。
Objective:To improve and optimize the determination methods for 15 chemical sun-screening agents in cosmetics by HPLC described in Hygienic Standard for Cosmetics (2007 edition) to enhance the quality control of limited chemical sun-screening a-gents in cosmetics. Methods:The samples were extracted using the improved mixed liquor. The detection was performed on an Accur-asilC18(250mm×4.6mm,5um)analyticalcolumn,themobilephasewasmethanol-tetrahydrofuran-water(adjustingpHto2.60 with acetic acid) with gradient elution, the flow rate was 1. 0 ml·min-1 , the column temperature was 25℃,and the detection wave-length was 311nm. Results:The method showed good linear relationship and the correlation coefficient was above 0. 999 5, the detec-tion limit was 1. 9-18. 7 ng, and the average recovery was within the range of 97. 5%-113. 7% with RSD of 0. 85%-1. 56%(n=9). Conclusion:The improved method is reliable, which is the effective optimization and complement for the determination methods of chemical sun-screening agents in cosmetic described in Hygienic Standard for Cosmetics (2007 edition).
