中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
11期
1975-1976
,共2页
治咳川贝枇杷露%熊果酸%高效液相色谱法%含量测定
治咳川貝枇杷露%熊果痠%高效液相色譜法%含量測定
치해천패비파로%웅과산%고효액상색보법%함량측정
Zhike Chuanbei Pipa syrups%Ursolic acid%HPLC%Determination
目的：建立治咳川贝枇杷露中熊果酸含量测定方法。方法：采用高效液相色谱法,使用 Cosmosil-C18(250 mm ×4.6 mm,5μm )为色谱柱；甲醇∶水∶冰醋酸∶三乙胺(90∶10∶0.03∶0.06)为流动相；流速为1.0 ml·min-1；柱温为室温(25℃),检测波长为215 nm。结果：样品中熊果酸在16~80μg·ml-1浓度范围内呈线性关系, r=0.9995(n=5),加样回收率为98.67%,RSD为1.22%(n=6)。结论：本法简便、准确、可靠、专属性强、重复性好、可作为治咳川贝枇杷露中熊果酸的含量测定。
目的：建立治咳川貝枇杷露中熊果痠含量測定方法。方法：採用高效液相色譜法,使用 Cosmosil-C18(250 mm ×4.6 mm,5μm )為色譜柱；甲醇∶水∶冰醋痠∶三乙胺(90∶10∶0.03∶0.06)為流動相；流速為1.0 ml·min-1；柱溫為室溫(25℃),檢測波長為215 nm。結果：樣品中熊果痠在16~80μg·ml-1濃度範圍內呈線性關繫, r=0.9995(n=5),加樣迴收率為98.67%,RSD為1.22%(n=6)。結論：本法簡便、準確、可靠、專屬性彊、重複性好、可作為治咳川貝枇杷露中熊果痠的含量測定。
목적：건립치해천패비파로중웅과산함량측정방법。방법：채용고효액상색보법,사용 Cosmosil-C18(250 mm ×4.6 mm,5μm )위색보주；갑순∶수∶빙작산∶삼을알(90∶10∶0.03∶0.06)위류동상；류속위1.0 ml·min-1；주온위실온(25℃),검측파장위215 nm。결과：양품중웅과산재16~80μg·ml-1농도범위내정선성관계, r=0.9995(n=5),가양회수솔위98.67%,RSD위1.22%(n=6)。결론：본법간편、준학、가고、전속성강、중복성호、가작위치해천패비파로중웅과산적함량측정。
Objective:To establish a method to determine the content of ursolic acid in Zhike Chuanbei Pipa syrups. Methods:An HPLC method was used with a Cosmosil-C18 column(250 mm × 4. 6 mm, 5 μm). The mobile phase was methanol-water-glacial acetic acid-triethylamine(90∶10∶0. 03∶0. 06) with a flow rate of 1. 0 ml·min-1 , the column temperature was 25℃, and the detec-tion wavelength was set at 215nm. Results:The linear range of ursolic acid was 16-80μg·ml-1(r=0. 999 5, n=5), and the aver-age recovery was 98. 67℅ (RSD=1. 22, n=6). Conclusion:The method is simple, accurate and reliable with good specificity and reproducibility, and it can be used in the quantitative determination of ursolic acid in Zhike Chuanbei Pipa syrups.
