中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
11期
1963-1964
,共2页
余华丽%王伟影%毛菊华%陈张金
餘華麗%王偉影%毛菊華%陳張金
여화려%왕위영%모국화%진장금
畲药%老虎吊%齐墩果酸%高效液相色谱法
畬藥%老虎弔%齊墩果痠%高效液相色譜法
여약%로호조%제돈과산%고효액상색보법
Traditional She medicine%Radix of Aralia chinensis L.%Oleanolic acid%HPLC
目的：建立HPLC法测定畲药老虎吊中齐墩果酸的含量。方法：色谱柱为Waters XBridge C18(250 mm ×4.6 mm,5μm)；流动相为甲醇-0.1 mol·L-1乙酸铵溶液(83∶17)；流速为1.0 ml·min-1；柱温为20℃；检测波长为210 nm；进样量为10μl。结果：老虎吊中齐墩果酸与其他组分分离良好,线性范围为72.52~725.2μg·ml-1(r=1.0000)；平均加样回收率为98.05%(RSD=1.89%,n=6)。结论：该方法简便易行、准确、重复性好,可用于畲药老虎吊中齐墩果酸的含量测定。
目的：建立HPLC法測定畬藥老虎弔中齊墩果痠的含量。方法：色譜柱為Waters XBridge C18(250 mm ×4.6 mm,5μm)；流動相為甲醇-0.1 mol·L-1乙痠銨溶液(83∶17)；流速為1.0 ml·min-1；柱溫為20℃；檢測波長為210 nm；進樣量為10μl。結果：老虎弔中齊墩果痠與其他組分分離良好,線性範圍為72.52~725.2μg·ml-1(r=1.0000)；平均加樣迴收率為98.05%(RSD=1.89%,n=6)。結論：該方法簡便易行、準確、重複性好,可用于畬藥老虎弔中齊墩果痠的含量測定。
목적：건립HPLC법측정여약로호조중제돈과산적함량。방법：색보주위Waters XBridge C18(250 mm ×4.6 mm,5μm)；류동상위갑순-0.1 mol·L-1을산안용액(83∶17)；류속위1.0 ml·min-1；주온위20℃；검측파장위210 nm；진양량위10μl。결과：로호조중제돈과산여기타조분분리량호,선성범위위72.52~725.2μg·ml-1(r=1.0000)；평균가양회수솔위98.05%(RSD=1.89%,n=6)。결론：해방법간편역행、준학、중복성호,가용우여약로호조중제돈과산적함량측정。
Objective:To establish an HPLC method for the determination of oleanolic acid in She medicine radix of Aralia chinen-sis L. . Methods:The HPLC analysis was performed on a Waters XBridge-C18 (250 mm × 4. 6 mm, 5 μm) column. The mobile phase was methanol-0. 1 mol·L-1 ammonium acetate solution (83∶17) and the flow rate was 1. 0 ml·min-1 . The detection wavelength was 210 nm, the column temperature was 20 ℃, and the injection volume was 10 μl. Results:Oleanolic acid in radix of Aralia chinensis L. had a good separation from the other components, a good linearity was obtained within the range of 72. 52-725. 2 μg·ml-1 ( r=1. 000 0), and the average recovery was 98. 05%(RSD=1. 89%, n=6). Conclusion:The method is simple, accurate, reproducible and applicable in the assay of oleanolic acid in radix of Aralia chinensis L. .
