中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
11期
1879-1882
,共4页
氯霉素%水杨酸%复方氯霉素醇溶液%含量测定%有关物质
氯黴素%水楊痠%複方氯黴素醇溶液%含量測定%有關物質
록매소%수양산%복방록매소순용액%함량측정%유관물질
Chloramphenicol%Salicylic acid%Compound chloramphenicol alcoholic solutions%Determination%Related substances
目的：建立高效液相色谱法测定复方氯霉素醇溶液中氯霉素、水杨酸含量及其有关物质的检查方法。方法：色谱柱为 Eclipse XDB-C18柱(150 mm ×4.6 mm,5μm),流动相为0.8%冰醋酸溶液-乙腈(60∶40),流速为1.0 ml·min-1,柱温25℃,检测波长290 nm(其中有关物质检查检测波长为272 nm),进样量为10μl,外标法计算含量。结果：氯霉素及水杨酸与有关检查物质能完全分离,氯霉素的线性范围为14.88~297.60μg·ml-1(r=0.9999),水杨酸的线性范围为9.72~194.40μg· ml-1-1(r=1.0000)；氯霉素与水杨酸的平均回收率分别为101.18%,99.78%,RSD分别为0.82%,0.27%(n=9)。结论：该方法操作简便、重复性好、结果准确,可用于同时测定复方氯霉素醇溶液中两种主要成分的含量与有关杂质的检查。
目的：建立高效液相色譜法測定複方氯黴素醇溶液中氯黴素、水楊痠含量及其有關物質的檢查方法。方法：色譜柱為 Eclipse XDB-C18柱(150 mm ×4.6 mm,5μm),流動相為0.8%冰醋痠溶液-乙腈(60∶40),流速為1.0 ml·min-1,柱溫25℃,檢測波長290 nm(其中有關物質檢查檢測波長為272 nm),進樣量為10μl,外標法計算含量。結果：氯黴素及水楊痠與有關檢查物質能完全分離,氯黴素的線性範圍為14.88~297.60μg·ml-1(r=0.9999),水楊痠的線性範圍為9.72~194.40μg· ml-1-1(r=1.0000)；氯黴素與水楊痠的平均迴收率分彆為101.18%,99.78%,RSD分彆為0.82%,0.27%(n=9)。結論：該方法操作簡便、重複性好、結果準確,可用于同時測定複方氯黴素醇溶液中兩種主要成分的含量與有關雜質的檢查。
목적：건립고효액상색보법측정복방록매소순용액중록매소、수양산함량급기유관물질적검사방법。방법：색보주위 Eclipse XDB-C18주(150 mm ×4.6 mm,5μm),류동상위0.8%빙작산용액-을정(60∶40),류속위1.0 ml·min-1,주온25℃,검측파장290 nm(기중유관물질검사검측파장위272 nm),진양량위10μl,외표법계산함량。결과：록매소급수양산여유관검사물질능완전분리,록매소적선성범위위14.88~297.60μg·ml-1(r=0.9999),수양산적선성범위위9.72~194.40μg· ml-1-1(r=1.0000)；록매소여수양산적평균회수솔분별위101.18%,99.78%,RSD분별위0.82%,0.27%(n=9)。결론：해방법조작간편、중복성호、결과준학,가용우동시측정복방록매소순용액중량충주요성분적함량여유관잡질적검사。
Objective:To establish an HPLC method to determine the content of chloramphenicol, salicylic acid and the related substances in compound chloramphenicol alcoholic solutions. Methods:The analytical column was Agilent Eclipse XDB-C18 (150 mm × 4. 6 mm,5 μm), 0. 8% acetic acid solution- acetonitrile(60∶40) was used as the mobile phase with the flow rate of 1. 0 ml· min-1 , the detection wavelength was 290nm for chloramphenicol and salicylic acid, and 272nm for the related substances, the column temperature was 25℃,and the injection volume was 10 μl. Results: Chloramphenicol and salicylic acid were completely separated from the related substances. The linear relationship of chloramphenicol ranged from 14. 88 to 297. 60μg·ml-1(r=0. 999 9), and the average recovery was 101. 18% with RSD of 0. 82%(n=9). The linear relationship of salicylic acid ranged from 9. 72 to 194. 40μg· ml-1(r=1. 000 0), and the average recovery was 99. 78% with RSD of 0. 27%(n=9). Conclusion:The method is good in resolu-tion, reproducibility and sensitivity. It is suitable for the determination of the two components and related substances in compound chloramphenicol alcoholic solutions.
