中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
11期
1873-1876
,共4页
王嘉林%程芳%赵静%郭润勤
王嘉林%程芳%趙靜%郭潤勤
왕가림%정방%조정%곽윤근
三七接骨丸%高效液相色谱法%人参皂苷Rg1%人参皂苷Rb1%三七皂苷R1
三七接骨汍%高效液相色譜法%人參皂苷Rg1%人參皂苷Rb1%三七皂苷R1
삼칠접골환%고효액상색보법%인삼조감Rg1%인삼조감Rb1%삼칠조감R1
Sanqi Jiegu pills%HPLC%Gensenoside Rg1%Gensenoside Rb1%Notogensenoside R1
目的：建立三七接骨丸中三萜类皂苷(人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1)的HPLC含量测定方法并考察其处方稳定性。方法：色谱柱：COSMOSIL 5 C18 MS-II(250 mm ×4.6 mm,5μm)；流动相：水-乙腈,梯度洗脱；流速：1.0 ml·min-1；检测波长：203 nm,进样量10μl；柱温为30℃。同时考察在不同温度下制粒,三七中皂苷含量的变化。结果：三七皂苷R1、人参皂苷Rg1、和人参皂苷Rb1分别在0.2118~2.6480μg·ml-1(r =0.9997),0.5661~7.0760μg·ml-1(r =0.9997),0.3172~3.9645μg·ml-1(r=0.9997)之间线性范围良好。平均回收率：三七皂苷R1为98.2%(RSD=0.82%,n=6),人参皂苷Rg1为98.1%(RSD=0.72%,n=6),人参皂苷Rb1为98.1%(RSD=0.59%,n=6)。三种皂苷随着温度增加降解也随之增加。结论：该方法简单、迅速,耐用,能够控制三七接骨丸的质量,同时建议三七接骨丸制粒干燥温度控制在60℃以下。
目的：建立三七接骨汍中三萜類皂苷(人參皂苷Rg1、人參皂苷Rb1、三七皂苷R1)的HPLC含量測定方法併攷察其處方穩定性。方法：色譜柱：COSMOSIL 5 C18 MS-II(250 mm ×4.6 mm,5μm)；流動相：水-乙腈,梯度洗脫；流速：1.0 ml·min-1；檢測波長：203 nm,進樣量10μl；柱溫為30℃。同時攷察在不同溫度下製粒,三七中皂苷含量的變化。結果：三七皂苷R1、人參皂苷Rg1、和人參皂苷Rb1分彆在0.2118~2.6480μg·ml-1(r =0.9997),0.5661~7.0760μg·ml-1(r =0.9997),0.3172~3.9645μg·ml-1(r=0.9997)之間線性範圍良好。平均迴收率：三七皂苷R1為98.2%(RSD=0.82%,n=6),人參皂苷Rg1為98.1%(RSD=0.72%,n=6),人參皂苷Rb1為98.1%(RSD=0.59%,n=6)。三種皂苷隨著溫度增加降解也隨之增加。結論：該方法簡單、迅速,耐用,能夠控製三七接骨汍的質量,同時建議三七接骨汍製粒榦燥溫度控製在60℃以下。
목적：건립삼칠접골환중삼첩류조감(인삼조감Rg1、인삼조감Rb1、삼칠조감R1)적HPLC함량측정방법병고찰기처방은정성。방법：색보주：COSMOSIL 5 C18 MS-II(250 mm ×4.6 mm,5μm)；류동상：수-을정,제도세탈；류속：1.0 ml·min-1；검측파장：203 nm,진양량10μl；주온위30℃。동시고찰재불동온도하제립,삼칠중조감함량적변화。결과：삼칠조감R1、인삼조감Rg1、화인삼조감Rb1분별재0.2118~2.6480μg·ml-1(r =0.9997),0.5661~7.0760μg·ml-1(r =0.9997),0.3172~3.9645μg·ml-1(r=0.9997)지간선성범위량호。평균회수솔：삼칠조감R1위98.2%(RSD=0.82%,n=6),인삼조감Rg1위98.1%(RSD=0.72%,n=6),인삼조감Rb1위98.1%(RSD=0.59%,n=6)。삼충조감수착온도증가강해야수지증가。결론：해방법간단、신속,내용,능구공제삼칠접골환적질량,동시건의삼칠접골환제립간조온도공제재60℃이하。
Objective:To establish an HPLC method for the determination gensenoside Rg1 , Rb1 and notogensenoside R1 in Sanqi Jiegu pills and to investigate the stability of saponins in the granulation process. Methods:The separation was performes on a COSMO-SIL 5 C18 MS-II(250 mm × 4. 6 mm,5μm)column with gradient elution using water and acetonitrile as the mobile phase, the flow rate was 1. 0 ml·min-1 , the detection wavelength was 203nm, the column temperature was 30℃, and the injection volume was 10 μl. The content change of triterpene saponins in Sanqi Jiegu pills prepared by the granulation process at different drying temperature was al-so investigated. Results:The linearity of notogenoside R1 , gensenoside Rg1 and gensenoside Rb1 was good within the range of 0. 211 8-2.648 0 μg·ml-1(r=0.999 7),0.566 1-7.076 0 μg·ml-1(r=0.999 7) and 0.317 2-3.964 5μg·ml-1(r=0.999 7) with the average recovery of 98. 2%(RSD=0. 82%,n=6), 98. 1%(RSD=0. 72%,n=6) and 98. 1%(RSD=0. 59%,n=6),respectively. The content of saponins in Saqi Jiegu pills was decreased obviously with the increase of the drying temperature. Conclusion:The meth-od is simple, quick and reliable, and can be applied in the quality control of Sanqi Jiegu pills. It is recommended that the drying tem-perature in the granulation process should be controlled below 60℃.
