CAJ | 학술논문

目的：建立超高效液相色谱法( UPLC)同时测定穿心莲内酯磺化物中穿心莲内酯磺化物E、穿心莲内酯磺化物F含量。方法：色谱柱：Shimadzu Shim-pack XR-ODSⅢ(2.0 m ×75 mm,1.6μm)；流动相：乙睛(A),磷酸二氢钾缓冲液(B：每1000 ml水中加入磷酸二氢钾1.361 g与庚烷-1-磺酸钠1.0 g)梯度洗脱；流速：0.4 ml·min-1；检测波长：225 nm；柱温：30℃。结果：穿心莲内酯磺化物E在4.39~43.88 g·ml-1(r=1.0000)、穿心莲内酯磺化物F在4.43~44.32μg·ml-1(r=1.0000)范围内,呈良好的线性关系。穿心莲内酯磺化物E平均回收率为98.71%, RSD=1.41%(n=6)；穿心莲内酯磺化物F平均回收率为99.29%,RSD=0.76%(n=6)。结论：本法重复性好、灵敏度高、定量准确,可用于穿心莲内酯磺化物的质量控制。
목적：건립초고효액상색보법( UPLC)동시측정천심련내지광화물중천심련내지광화물E、천심련내지광화물F함량。방법：색보주：Shimadzu Shim-pack XR-ODSⅢ(2.0 m ×75 mm,1.6μm)；류동상：을정(A),린산이경갑완충액(B：매1000 ml수중가입린산이경갑1.361 g여경완-1-광산납1.0 g)제도세탈；류속：0.4 ml·min-1；검측파장：225 nm；주온：30℃。결과：천심련내지광화물E재4.39~43.88 g·ml-1(r=1.0000)、천심련내지광화물F재4.43~44.32μg·ml-1(r=1.0000)범위내,정량호적선성관계。천심련내지광화물E평균회수솔위98.71%, RSD=1.41%(n=6)；천심련내지광화물F평균회수솔위99.29%,RSD=0.76%(n=6)。결론：본법중복성호、령민도고、정량준학,가용우천심련내지광화물적질량공제。
Objective:To establish an UPLC method for the simultaneous determination of andrographolide sulfonate E and an-drographolide sulfonate F in andrographolide sulfonates. Methods: The sample was separated on a Shimadzu Shim-pack XR-ODSⅢcolumn (2. 0 m × 75 mm, 1. 6 μm) with acetonitrile( A) and potassium dihydrogen phosphate buffer solution( B:1. 361 g potassium dihydrogen phosphate and 1. 0g heptane-1-sodium sulfonate were dissolved in 1 000 ml water) as the mobile phase with gradient elu-tion, and the flow rate was 0. 4 ml·min-1 ,the column temperature was 30℃, and the detection wavelength was set at 225nm. Re-sults:The linear range of andrographolide sulfonate E was 4. 39-43. 88μg·ml-1(r=1. 000 0) and the average recovery was 98. 71%(RSD=1. 41%, n=6). The linear range of andrographolide sulfonate F was 4. 43-44. 32 g·ml-1(r=1. 000 0) and the average re-covery was 99. 29%(RSD=0. 76%, n=6). Conclusion:The method is reproducible, sensitive and accurate, which can be used in the quality control of andrographolide sulfonates.