中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
11期
1808-1810
,共3页
毛菊华%王伟影%余乐%程科军
毛菊華%王偉影%餘樂%程科軍
모국화%왕위영%여악%정과군
畲药%食凉茶%芦丁%槲皮素%山柰酚%高效液相色谱法
畬藥%食涼茶%蘆丁%槲皮素%山柰酚%高效液相色譜法
여약%식량다%호정%곡피소%산내분%고효액상색보법
Traditional She medicine%Shi Liang Cha%Rutin%Quercetin%Kaempferol%HPLC
目的：建立同时测定畲药食凉茶中三种黄酮类成分含量的方法。方法：采用高效液相色谱法测定,色谱柱为Agilent Zorbax SB-C18色谱柱(250 mm ×4.6 mm,5μm)；流动相为乙腈-0.1%磷酸溶液,梯度洗脱；流速为1.0 ml·min-1,柱温30℃；检测波长360 nm。结果：芦丁、槲皮素和山柰酚的线性范围分别为0.0409~1.6370 mg·ml-1(r=0.9992),0.44~88.00μg ·ml-1(r=0.9998),0.41~77.63μg·ml-1(r=0.9992)；平均加样回收率分别为99.35%(RSD=1.64%),101.14%(RSD=1.88%),99.69%(RSD=1.92%)。结论：该方法简便易行、快速准确、重复性好,可用于同时测定食凉茶中的芦丁、槲皮素和山柰酚的含量。
目的：建立同時測定畬藥食涼茶中三種黃酮類成分含量的方法。方法：採用高效液相色譜法測定,色譜柱為Agilent Zorbax SB-C18色譜柱(250 mm ×4.6 mm,5μm)；流動相為乙腈-0.1%燐痠溶液,梯度洗脫；流速為1.0 ml·min-1,柱溫30℃；檢測波長360 nm。結果：蘆丁、槲皮素和山柰酚的線性範圍分彆為0.0409~1.6370 mg·ml-1(r=0.9992),0.44~88.00μg ·ml-1(r=0.9998),0.41~77.63μg·ml-1(r=0.9992)；平均加樣迴收率分彆為99.35%(RSD=1.64%),101.14%(RSD=1.88%),99.69%(RSD=1.92%)。結論：該方法簡便易行、快速準確、重複性好,可用于同時測定食涼茶中的蘆丁、槲皮素和山柰酚的含量。
목적：건립동시측정여약식량다중삼충황동류성분함량적방법。방법：채용고효액상색보법측정,색보주위Agilent Zorbax SB-C18색보주(250 mm ×4.6 mm,5μm)；류동상위을정-0.1%린산용액,제도세탈；류속위1.0 ml·min-1,주온30℃；검측파장360 nm。결과：호정、곡피소화산내분적선성범위분별위0.0409~1.6370 mg·ml-1(r=0.9992),0.44~88.00μg ·ml-1(r=0.9998),0.41~77.63μg·ml-1(r=0.9992)；평균가양회수솔분별위99.35%(RSD=1.64%),101.14%(RSD=1.88%),99.69%(RSD=1.92%)。결론：해방법간편역행、쾌속준학、중복성호,가용우동시측정식량다중적호정、곡피소화산내분적함량。
Objective: To establish an HPLC method for the simultaneous determination of three flavonoids in Shi Liang Cha. Methods:HPLC was applied with the chromatographic conditions as follows: the chromatographic column was Agilent Zorbax SB-C18 (250 mm × 4. 6 mm, 5μm) at 30℃;acetonitrile-0. 1% H3 PO4 solution was used as the mobile phase with gradient elution; the flow rate was 1. 0 ml·min-1, and the detection wavelength was 360nm. Results: Good linearity of rutin, quercetin and kaempferol was within the range of 0.0409-1.637 0mg·ml-1(r=0.999 2), 0.44-88.00μg·ml-1(r=0.999 8) and 0.41-77.63μg·ml-1(r=0. 999 2), respectively; the average recovery was 99.35%(RSD =1. 64%),101. 14% (RSD =1. 88%) and 99. 69% (RSD =1. 92%) , respectively. Conclusion:The method is simple, accurate and repeatable, and suitable for the simultaneous determination of rutin, quercetin and kaempferol in Shi Liang Cha.
