CAJ | 학술논문

目的：观察玉郎伞多糖( YLSP)对环磷酰胺( CTX)致小鼠肝损伤的治疗作用并探讨其作用机制。方法：将小鼠随机分为正常对照组( NC),肝损伤模型组( CTX),阳性对照组(联苯双酯,BPDC),YLSP(玉郎伞多糖)高、中、低剂量组。除正常对照组外,其余各组小鼠每天腹腔注射CTX 40 mg·kg-1,共7 d,建立小鼠肝损伤模型。造模后连续灌胃7 d,观察YLSP对小鼠血清谷氨酸氨基转移酶( ALT)、天冬氨酸氨基转移酶( AST)、谷胱甘肽( GSH)、谷胱甘肽过氧化物酶( GSH-Px)、超氧化物歧化酶( SOD)和丙二醛( MDA)活性,苏木精-伊红( HE)染色观察小鼠肝脏组织病理学改变。结果：与模型组比较,YLSP各剂量组均能降低小鼠血清中ALT、AST活性及肝组织中MDA的含量；同时能增加肝组织中GSH含量及SOD和GSH-Px活性( P<0.05或P<0.01)；HE染色结果显示YLSP能减轻CTX所致的肝组织变性、坏死程度,可减轻小鼠肝组织病理损伤程度。结论：YLSP对CTX所致小鼠肝损伤具有良好的保护作用。
목적：관찰옥랑산다당( YLSP)대배린선알( CTX)치소서간손상적치료작용병탐토기작용궤제。방법：장소서수궤분위정상대조조( NC),간손상모형조( CTX),양성대조조(련분쌍지,BPDC),YLSP(옥랑산다당)고、중、저제량조。제정상대조조외,기여각조소서매천복강주사CTX 40 mg·kg-1,공7 d,건립소서간손상모형。조모후련속관위7 d,관찰YLSP대소서혈청곡안산안기전이매( ALT)、천동안산안기전이매( AST)、곡광감태( GSH)、곡광감태과양화물매( GSH-Px)、초양화물기화매( SOD)화병이철( MDA)활성,소목정-이홍( HE)염색관찰소서간장조직병이학개변。결과：여모형조비교,YLSP각제량조균능강저소서혈청중ALT、AST활성급간조직중MDA적함량；동시능증가간조직중GSH함량급SOD화GSH-Px활성( P<0.05혹P<0.01)；HE염색결과현시YLSP능감경CTX소치적간조직변성、배사정도,가감경소서간조직병리손상정도。결론：YLSP대CTX소치소서간손상구유량호적보호작용。
Objective:To observe the protective effect of Yulangsan polysaccharide ( YLSP) on liver injury induced by cyclophos-phamide(CTX) in mice. Methods:Liver injury induced by CTX in mice was used as the animal model and the mice were randomly di-vided into the normal group, CTX model group, biphenyldicarboxylate ( BPDC) group, YLSP group respectively with high, medium and low dose. Except the normal group, the other groups were injected with CTX, i. p. , for 7 days to make the model. Then the ani-mals in the YLSP groups were intragastrically administered with YLSP for 7 days. The activities of alanine aminotransferase( ALT) , as-partate aminotransferase( AST) in serum, malondialdehyde( MDA) , superoxide dismutase( SOD) , glutathione( GSH) and glutathione peroxidase ( GSH-Px) in liver tissue were investigated. Hematoxylin and eosin ( HE) stain was used to study the changes in hepatic tissue of the pathological mice. Results:Compared with the model group, YLSP could obviously reduce the activities of ALT, AST and the content of MDA, and increase the content of GSH, SOD and GSH-Px (P<0. 05 or P<0. 01). HE staining showed that YLSP had significant protective effect on liver injury induced by CTX. Conclusion:YLSP has protective effect on liver injury induced by CTX.