中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
11期
1793-1795,1796
,共4页
鱼藤素%Neuro-2A细胞%细胞存活率%caspase 3
魚籐素%Neuro-2A細胞%細胞存活率%caspase 3
어등소%Neuro-2A세포%세포존활솔%caspase 3
Deguelin%Neruo-2A cell%Cell viability%Caspase 3
目的：研究鱼藤素对小鼠神经母细胞瘤细胞Neuro-2A ( N2A)存活率及凋亡的影响。方法： N2A细胞以5×104· ml-1密度接种到细胞培养板中,1×10-8 mol·L-1鱼藤素处理后6,12,24,48,72 h等5个时间点上测定细胞存活率；加入鱼藤素0,1×10-11,1×10-10,1×10-9,1×10-8,1×10-7,1×10-6 mol·L-1等7个不同浓度干预48 h后测定细胞存活率。2×10-8 mol·L-1鱼藤素干预24 h,测定细胞匀浆中caspase 3活力。结果：5×104·ml-1 N2A细胞以含10%胎牛血清DMEM培养基培养,第48 h达到细胞生长曲线的峰值。1×10-8 mol·L-1鱼藤素干预24~72 h,时间依赖性显著降低N2A细胞存活率(P<0.05)；在鱼藤素干预48 h时间点上,1×10-8~1×10-6·mol·L-1浓度范围内剂量依赖性可显著降低N2A细胞存活率(P<0.05),其IC50=1.6×10-8 mol·L-1。鱼藤素作用于N2A细胞24 h可显著提高caspase 3活力,约达到对照组的5.6倍。结论：鱼藤素可时间依赖性且剂量依赖性降低N2A细胞存活率,可能与增加caspase 3活力有关。
目的：研究魚籐素對小鼠神經母細胞瘤細胞Neuro-2A ( N2A)存活率及凋亡的影響。方法： N2A細胞以5×104· ml-1密度接種到細胞培養闆中,1×10-8 mol·L-1魚籐素處理後6,12,24,48,72 h等5箇時間點上測定細胞存活率；加入魚籐素0,1×10-11,1×10-10,1×10-9,1×10-8,1×10-7,1×10-6 mol·L-1等7箇不同濃度榦預48 h後測定細胞存活率。2×10-8 mol·L-1魚籐素榦預24 h,測定細胞勻漿中caspase 3活力。結果：5×104·ml-1 N2A細胞以含10%胎牛血清DMEM培養基培養,第48 h達到細胞生長麯線的峰值。1×10-8 mol·L-1魚籐素榦預24~72 h,時間依賴性顯著降低N2A細胞存活率(P<0.05)；在魚籐素榦預48 h時間點上,1×10-8~1×10-6·mol·L-1濃度範圍內劑量依賴性可顯著降低N2A細胞存活率(P<0.05),其IC50=1.6×10-8 mol·L-1。魚籐素作用于N2A細胞24 h可顯著提高caspase 3活力,約達到對照組的5.6倍。結論：魚籐素可時間依賴性且劑量依賴性降低N2A細胞存活率,可能與增加caspase 3活力有關。
목적：연구어등소대소서신경모세포류세포Neuro-2A ( N2A)존활솔급조망적영향。방법： N2A세포이5×104· ml-1밀도접충도세포배양판중,1×10-8 mol·L-1어등소처리후6,12,24,48,72 h등5개시간점상측정세포존활솔；가입어등소0,1×10-11,1×10-10,1×10-9,1×10-8,1×10-7,1×10-6 mol·L-1등7개불동농도간예48 h후측정세포존활솔。2×10-8 mol·L-1어등소간예24 h,측정세포균장중caspase 3활력。결과：5×104·ml-1 N2A세포이함10%태우혈청DMEM배양기배양,제48 h체도세포생장곡선적봉치。1×10-8 mol·L-1어등소간예24~72 h,시간의뢰성현저강저N2A세포존활솔(P<0.05)；재어등소간예48 h시간점상,1×10-8~1×10-6·mol·L-1농도범위내제량의뢰성가현저강저N2A세포존활솔(P<0.05),기IC50=1.6×10-8 mol·L-1。어등소작용우N2A세포24 h가현저제고caspase 3활력,약체도대조조적5.6배。결론：어등소가시간의뢰성차제량의뢰성강저N2A세포존활솔,가능여증가caspase 3활력유관。
Objective:To investigate the effects of deguelin on the viability and apoptosis of Neuro-2A ( N2A) cells. Methods:The cell viability of N2A cells with the density of 5 × 104 ·ml-1 on the time points of 6, 12, 24, 48 and 72 hours after 1 × 10 -8 mol ·L-1 deguelin treatment using CCK-8 kits was determined, and that on the time point of 48 hours after 0, 1 × 10 -11 , 1 × 10 -10 , 1 × 10 -9 , 1 × 10 -8 , 1 × 10 -7 , 1 × 10 -6 or 5 × 10 -4 mol·L-1 deguelin treatment was also detected. The activity of caspase 3 was deter-mined in N2A cells treated by 2 × 10 -8 mol·L-1 deguelin for 24 hours. Results:N2A cells with the density of 5 × 104 ·ml-1 reached the peak level in the growth curve 48 hours after the incubation in DMEM medium with 10% fetal bovine serum. The cell viability of N2A cells was decreased after the treatment of 1 × 10 -8 mol·L-1 deguelin for 24-72 hours in a time-dependant manner or after the treatment of 1 × 10 -8 ~1 × 10 -6 mol·L-1 deguelin for 48 hours in a dose-dependant manner (P<0. 05) with IC50 of 1. 6 × 10 -8 mol ·L-1 . The activity of caspase 3 was increased after the treatment of 2 × 10 -8 mol·L-1 deguelin for 24 hours, which was 5. 6-fold of that in the control group. Conclusion: Cell viability of N2A cells is inhibited by deguelin in a time- and dose-dependent manner, which may be related to the activity increase of caspase 3.
