CAJ | 학술논문

目的：利用本实验室制备的嗜水气单胞菌灭活疫苗免疫银鲫，研究疫苗的免疫保护效果。方法：将银鲫随机分为3组：注射组、浸泡组和对照组进行试验，二次免疫后，分别对银鲫组织和血液样品进行组织病理学、血液免疫学研究以及免疫保护率的测定。结果：血液指标检测显示，注射组、浸泡组各项血液指标发生显著改变，银鲫各项免疫指标显著提高；组织病理学显示，攻毒后，对照组的肝、肾、脾等重要组织已发生病变，而注射组和浸泡组无明显的变化；注射组、浸泡组和对照组的免疫保护率分别为90％、60％、0％。结论：嗜水气单胞菌灭活疫苗对银鲫的细菌性出血病具有显著的保护作用。
목적：이용본실험실제비적기수기단포균멸활역묘면역은즉，연구역묘적면역보호효과。방법：장은즉수궤분위3조：주사조、침포조화대조조진행시험，이차면역후，분별대은즉조직화혈액양품진행조직병이학、혈액면역학연구이급면역보호솔적측정。결과：혈액지표검측현시，주사조、침포조각항혈액지표발생현저개변，은즉각항면역지표현저제고；조직병이학현시，공독후，대조조적간、신、비등중요조직이발생병변，이주사조화침포조무명현적변화；주사조、침포조화대조조적면역보호솔분별위90％、60％、0％。결론：기수기단포균멸활역묘대은즉적세균성출혈병구유현저적보호작용。
Objective:In order to find a safe and healthy way to control fish diseases caused by Aeromonas hydrophila,the immune effect of Aeromonas hydrophila inactivated vaccine on Carassius gibelio was studied.Methods: Three groups were randomly made by each of 10 fishes.Injection groups was made intraperitoneally ( i.p.) with 200 μl, and soaked group with 3 L volumes of inactivated vaccine suspensions respectively while the control group with same volumes of saline.Tissues and blood samples of carassius gibelio were collected after the second vaccination.Then the histopathological, immunological indicators and survival percentage were detected.Results:The relative survival percentage of two groups was 90%and 60%respectively.The serum enzymes of injection and soaked group showed significant differences contrasted with the control group.Histopathological studies revealed that the important tissues have been damaged obviously after the bacterium challenge, while the experiment groups were no damage.Conclusion:Therefore the inactivated vaccine could prevent carassius gibelio diseases that caused by A.hydrophila.