CAJ | 학술논문

目的：应用HLA-A*0201/CAP-1四聚体，分析HLA-A*0201＋CEA＋结直肠癌患者的外周血及癌旁肠系膜引流淋巴结的特异性CTLs的数量。方法：分离健康人（18例）PBMC和结直肠癌（23例）患者PBMC以及癌旁肠系膜引流淋巴结的淋巴细胞，应用流式细胞术，以HLA-A*0201/CAP-1和HLA-A*0201/FLUmp四聚体检测CAP-1和FLUmp特异性CTLs的频率。结果：在25例HLA-A*0201＋个体中，结直肠癌组和正常组外周血单个核细胞中HLA-A*0201/FLU四聚体＋CD8＋细胞的频率分别为（0.671±0.421）％和（0.564±0.408）％，两组之间无显著差异（P＝0.525）。结直肠癌组和正常组外周血单个核细胞中HLA-A*0201/CAP-1四聚体＋CD8＋细胞的频率分别为（2.409±2.385）％和（0.020±0.021）％，两组之间有显著差异（ P＝0.008）。结论：HLA-A*0201＋结直肠癌患者HLA-A*0201/CAP-1四聚体＋CD8＋细胞的频率升高，说明CEA特异性CTLs在结直肠癌患者的免疫监视功能中具有重要的作用。对存在针对肿瘤抗原的特异性CTLs却无法阻止肿瘤进展的具体机制需要更加深入的研究。
목적：응용HLA-A*0201/CAP-1사취체，분석HLA-A*0201＋CEA＋결직장암환자적외주혈급암방장계막인류림파결적특이성CTLs적수량。방법：분리건강인（18례）PBMC화결직장암（23례）환자PBMC이급암방장계막인류림파결적림파세포，응용류식세포술，이HLA-A*0201/CAP-1화HLA-A*0201/FLUmp사취체검측CAP-1화FLUmp특이성CTLs적빈솔。결과：재25례HLA-A*0201＋개체중，결직장암조화정상조외주혈단개핵세포중HLA-A*0201/FLU사취체＋CD8＋세포적빈솔분별위（0.671±0.421）％화（0.564±0.408）％，량조지간무현저차이（P＝0.525）。결직장암조화정상조외주혈단개핵세포중HLA-A*0201/CAP-1사취체＋CD8＋세포적빈솔분별위（2.409±2.385）％화（0.020±0.021）％，량조지간유현저차이（ P＝0.008）。결론：HLA-A*0201＋결직장암환자HLA-A*0201/CAP-1사취체＋CD8＋세포적빈솔승고，설명CEA특이성CTLs재결직장암환자적면역감시공능중구유중요적작용。대존재침대종류항원적특이성CTLs각무법조지종류진전적구체궤제수요경가심입적연구。
Objective:To analyze the frequencies of HLA-A*0201 restricted CEA-specific CD8+T cells, HLA-A*0201/FLUmp tetramer and HLA-A*0201/CAP-1 tetramer were applied in patients with colorectal cancer.Methods: Lymphocytes from peripheral blood and lymph node,1×106 cells/ml,were incubated with 1μg HLA-A*0201/peptide tetramers and anti-CD8 for 1 h at 25 coseperately.The cells were then washed in PBS.Next,the cells were illuminated by detecting frequencies of FLUmp-specific CD8+T cells and CAP-1-specific CD8+T cells with flow cytometry.Results: HLA-A*0201/peptide were used to detect CAP-1 or FLUmp-specific CD8+T cells,which were analyzed either healthy individuals or patients with colorectal cancer.We did not find differences in average frequencies of FLUmp-specific CD8+T cells between 11 HLA-A*0201+patients with colorectal cancer and 14 HLA-A*0201+healthy individuals [ ( 0.671 ±0.421 )%, ( 0.564 ±0.408 )%].But the frequencies of CAP-1-specific CD8+T cells of HLA-A*0201+patients with colorectal cancer showed higher than HLA-A*0201+healthy individuals [ ( 2.409 ± 2.385 )%, ( 0.020 ± 0.021)%respectively],which was statistically significant(P=0.008).Conclusion:The frequencies of CAP-1-specific CD8+T cells in PBMC from peripheral blood and lymph node of HLA-A*0201+patients were increased,showed CEA-specific CTs has a vital role in colorectal cancer.