CAJ | 학술논문

目的：通过观察姜黄素对佐剂性关节炎（ Adjuvant arthritis， AA ）模型大鼠滑膜病理、滑膜骨保护素（ Osteoprotegerin，OPG）、核因子κB受体活化因子配体（ Receptor activator for nuclear factor-κB ligand，RANKL）表达的影响，探讨其防治类风湿关节炎（ Rheumatoid arthritis，RA）可能作用机制。方法：雄性SD大鼠以弗氏完全佐剂造模，分为模型组与姜黄素组，另有正常组，d 28处死所有大鼠行滑膜常规病理HE染色，并用Western blot法来检测滑膜RANKL、OPG蛋白的表达。结果：模型组大鼠滑膜炎性细胞浸润、纤维细胞及滑膜细胞的增生较正常组均明显增加（P＜0.01），而姜黄素组的大鼠滑膜组织病理较模型组显著改善（ P＜0.01）；姜黄素组大鼠的滑膜RANKL表达显著低于模型组，滑膜OPG表达显著高于模型组， RANKL/OPG比值显著低于模型组（ P＜0.01）。结论：姜黄素不仅改善AA大鼠关节滑膜病理学损伤，还可调节滑膜RANKL/OPG的比值。因此姜黄素可能通过调节OPG/RANKL系统来发挥对AA大鼠的治疗作用。
목적：통과관찰강황소대좌제성관절염（ Adjuvant arthritis， AA ）모형대서활막병리、활막골보호소（ Osteoprotegerin，OPG）、핵인자κB수체활화인자배체（ Receptor activator for nuclear factor-κB ligand，RANKL）표체적영향，탐토기방치류풍습관절염（ Rheumatoid arthritis，RA）가능작용궤제。방법：웅성SD대서이불씨완전좌제조모，분위모형조여강황소조，령유정상조，d 28처사소유대서행활막상규병리HE염색，병용Western blot법래검측활막RANKL、OPG단백적표체。결과：모형조대서활막염성세포침윤、섬유세포급활막세포적증생교정상조균명현증가（P＜0.01），이강황소조적대서활막조직병리교모형조현저개선（ P＜0.01）；강황소조대서적활막RANKL표체현저저우모형조，활막OPG표체현저고우모형조， RANKL/OPG비치현저저우모형조（ P＜0.01）。결론：강황소불부개선AA대서관절활막병이학손상，환가조절활막RANKL/OPG적비치。인차강황소가능통과조절OPG/RANKL계통래발휘대AA대서적치료작용。
Objective:By observing the effects of curcumin on synovial pathology, synovial osteoprotegerin and receptor activator of nuclear factor κB ligand expression factor of adjuvant arthritis rat models, we try to explore the possible mechanism of prevention and treatment of rheumatoid arthritis.Methods: There were three groups, the normal group, the model group and the curcumin group.We made adjuvant arthritis rat models with Freund′s complete adjuvant,and they were divided into the model group and the curcumin group.On d 28 after modeling,all rats were killed.All synovial membranes were sent for routine histological evaluation with HE staining.And synovial RANKL and OPG protein expression were measured by Western blot.Results: Curcumin could influence synovium pathology change of adjuvant arthritis rats,the pathological picture showed that infiltration of joint synovial cell,pro-liferation of synovial fibroblasts in model group rats were significantly increased,compared with normal group( P<0.01).Pathology of rats synovial tissue in rats of curcumin group were significantly improved, compared with that in rats of model group.Expression of RANKL,OPG protein in synovial tissue of curcumin group,based on pairwise comparison between with model group,the results were statistically significant( P<0.01 ).Synovial RANKL/OPG ratio were significantly lower than model group ( P<0.01 ).Conclusion:Curcumin can improve synovial pathology damage of adjuvant arthritis rats,curcumin may exert a protective effect on arthritis rats by regulating OPG/RANKL signaling pathway.