CAJ | 학술논문

目的：研究在3T3-L1细胞中G蛋白偶联受体120（GPR120）与葡萄糖转运蛋白4（GLUT4）的关系。方法：诱导3T3-L1细胞分化，RT-PCR检测GRP120 mRNA表达，油红O染色检测细胞内脂肪；采用siRNA技术下调3T3-L1细胞中GPR120的表达，软脂酸孵育3T3-L1细胞24 h后，用real-time PCR和Western blot方法检测3T3-L1细胞中GLUT4的表达水平的变化。结果：诱导3T3-L1细胞分化过程中GPR120 mRNA表达升高（P＜0.05），干扰GPR120表达导致3T3-L1细胞诱导产生的脂滴体积和数量明显减小。另外，干扰GPR120表达导致GLUT4 mRNA和蛋白表达水平下降（ P＜0.05）。结论：GPR120影响了胰岛素信号通路中GLUT4表达水平，推测其参与了胰岛素抵抗的发生。
목적：연구재3T3-L1세포중G단백우련수체120（GPR120）여포도당전운단백4（GLUT4）적관계。방법：유도3T3-L1세포분화，RT-PCR검측GRP120 mRNA표체，유홍O염색검측세포내지방；채용siRNA기술하조3T3-L1세포중GPR120적표체，연지산부육3T3-L1세포24 h후，용real-time PCR화Western blot방법검측3T3-L1세포중GLUT4적표체수평적변화。결과：유도3T3-L1세포분화과정중GPR120 mRNA표체승고（P＜0.05），간우GPR120표체도치3T3-L1세포유도산생적지적체적화수량명현감소。령외，간우GPR120표체도치GLUT4 mRNA화단백표체수평하강（ P＜0.05）。결론：GPR120영향료이도소신호통로중GLUT4표체수평，추측기삼여료이도소저항적발생。
Objective:To investigate the correlation between G protein-coupled receptor 120 ( GPR120 ) and glucose transporter 4 (GLUT4) in 3T3-L1 cells.Methods:3T3-l1 cells were induced for differentiation,GRP120 mRNA was detected by RT-PCR and Oil red O was used to determine fat expression.GPR120 expression was knocked down by a specific siRNA in 3T3-L1cells.3T3-L1 cell that were tranfected with GPR120 siRNA were incubated with palmitic acid for 24 hours.Then,real-time PCR and Western blot were used to detect the expression of GLUT4 mRNA and protein.Results: Induced differentiation led to GPR120 overexpression in 3T3-L1 cells ( P<0.05 ).GPR120 knockdown resulted in reduction of both lipid volume and number in 3T3-L1 cells.Furthermore,GPR120 knockdown decreased GLUT4 mRNA and protein levels in 3T3-L1 cells (P<0.05,respectively).Conclusion:GPR120 affects the expression level of GLUT4 in insulin signaling pathway,suggesting its involvement in the initiation of insulin resist-ance.