江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2014年
11期
99-101
,共3页
华文君%华再东%郑新民%毕延震%肖红卫%张立苹
華文君%華再東%鄭新民%畢延震%肖紅衛%張立蘋
화문군%화재동%정신민%필연진%초홍위%장립평
猪%转hFat-1基因%整合%拷贝数
豬%轉hFat-1基因%整閤%拷貝數
저%전hFat-1기인%정합%고패수
Pig%Transgene hFat-1%Integration%Copy number
对转人源化Fat-1基因猪的15头传代仔猪进行PCR检测,发现有7头阳性猪,其断奶后成活5头仔猪。对这5头仔猪进行Southern印迹杂交试验,确认3头仔猪整合有hFat-1基因。采用荧光实时定量PCR法测定原代转hFat-1基因公猪及3头阳性仔猪外源基因的拷贝数,分别是2、1、1、2个拷贝。转hFat-1基因猪能够将外源基因遗传到下代仔猪,但外源基因具有遗传不稳定的趋向。
對轉人源化Fat-1基因豬的15頭傳代仔豬進行PCR檢測,髮現有7頭暘性豬,其斷奶後成活5頭仔豬。對這5頭仔豬進行Southern印跡雜交試驗,確認3頭仔豬整閤有hFat-1基因。採用熒光實時定量PCR法測定原代轉hFat-1基因公豬及3頭暘性仔豬外源基因的拷貝數,分彆是2、1、1、2箇拷貝。轉hFat-1基因豬能夠將外源基因遺傳到下代仔豬,但外源基因具有遺傳不穩定的趨嚮。
대전인원화Fat-1기인저적15두전대자저진행PCR검측,발현유7두양성저,기단내후성활5두자저。대저5두자저진행Southern인적잡교시험,학인3두자저정합유hFat-1기인。채용형광실시정량PCR법측정원대전hFat-1기인공저급3두양성자저외원기인적고패수,분별시2、1、1、2개고패。전hFat-1기인저능구장외원기인유전도하대자저,단외원기인구유유전불은정적추향。
Fifteen heads of hFat-1 transgenic offspring piglets were detected by PCR, 7 heads of piglets were positive, and 5 heads of piglets survived after weaning.Southern blotting cross experiment confirmed that the exogenous gene hFat-1 had been in-tegrated into 3 piglets among these 5 piglets.The copy numbers of hFat-1 in primary hFat-1 transgenic boar and 3 positive piglets were detected by fluorescence real-time Q-PCR, and they were 2, 1, 1, and 2 copies respectively.The integration of exogenous gene in hFat-1 transgenic pigs could be inherited to F1 -generation piglets, but could not be stably inherited.