高等学校化学学报
高等學校化學學報
고등학교화학학보
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES
2014年
11期
2325-2330
,共6页
丁国斌%李彬春%郭轶%徐力
丁國斌%李彬春%郭軼%徐力
정국빈%리빈춘%곽질%서력
10-羟基喜树碱%光谱性质%抗肿瘤活性%细胞凋亡%细胞标记
10-羥基喜樹堿%光譜性質%抗腫瘤活性%細胞凋亡%細胞標記
10-간기희수감%광보성질%항종류활성%세포조망%세포표기
10-Hydroxycamptothecin%Spectral property%Antitumor activity%Apoptosis%Cell labeling
通过紫外-可见光谱、荧光光谱和红外光谱等方法研究了药物10-羟基喜树碱( HCPT)的光谱性质.采用溴化噻唑蓝四氮唑(MTT)法测定了HCPT对3种肿瘤细胞(HeLa, MCF-7和HT1080)的抗肿瘤活性,其IC50值分别为16.37,16.73和19.24μg/mL.测定了HCPT对正常细胞人胚肾细胞系HEK293T的生长抑制活性,最高抑制率达88.72%,表明正常细胞比3种肿瘤细胞对药物HCPT更敏感.以HeLa细胞为模型,利用Annexin V-FITC细胞凋亡检测试剂盒研究了HCPT的抗肿瘤作用机制,发现几乎所有细胞均同时被Annexin V-FITC和碘化丙啶( PI)染色,细胞膜为绿色,而细胞核为红色,表明HCPT诱导了HeLa细胞的晚期凋亡.通过荧光显微镜观察了HCPT在HeLa细胞中的分布,为其在细胞标记中的应用提供了科学依据.
通過紫外-可見光譜、熒光光譜和紅外光譜等方法研究瞭藥物10-羥基喜樹堿( HCPT)的光譜性質.採用溴化噻唑藍四氮唑(MTT)法測定瞭HCPT對3種腫瘤細胞(HeLa, MCF-7和HT1080)的抗腫瘤活性,其IC50值分彆為16.37,16.73和19.24μg/mL.測定瞭HCPT對正常細胞人胚腎細胞繫HEK293T的生長抑製活性,最高抑製率達88.72%,錶明正常細胞比3種腫瘤細胞對藥物HCPT更敏感.以HeLa細胞為模型,利用Annexin V-FITC細胞凋亡檢測試劑盒研究瞭HCPT的抗腫瘤作用機製,髮現幾乎所有細胞均同時被Annexin V-FITC和碘化丙啶( PI)染色,細胞膜為綠色,而細胞覈為紅色,錶明HCPT誘導瞭HeLa細胞的晚期凋亡.通過熒光顯微鏡觀察瞭HCPT在HeLa細胞中的分佈,為其在細胞標記中的應用提供瞭科學依據.
통과자외-가견광보、형광광보화홍외광보등방법연구료약물10-간기희수감( HCPT)적광보성질.채용추화새서람사담서(MTT)법측정료HCPT대3충종류세포(HeLa, MCF-7화HT1080)적항종류활성,기IC50치분별위16.37,16.73화19.24μg/mL.측정료HCPT대정상세포인배신세포계HEK293T적생장억제활성,최고억제솔체88.72%,표명정상세포비3충종류세포대약물HCPT경민감.이HeLa세포위모형,이용Annexin V-FITC세포조망검측시제합연구료HCPT적항종류작용궤제,발현궤호소유세포균동시피Annexin V-FITC화전화병정( PI)염색,세포막위록색,이세포핵위홍색,표명HCPT유도료HeLa세포적만기조망.통과형광현미경관찰료HCPT재HeLa세포중적분포,위기재세포표기중적응용제공료과학의거.
The spectral properties of 10-hydroxycamptothecin( HCPT) were investigated by UV-Vis spectro-photometer, fluorescence spectrophotometer and fourier transform infrared spectroscopy. The antiproliferative activities of HCPT against three tumor cell lines, human cervical can-cer cell line ( HeLa ) , human breast cancer cell line(MCF-7) and human fibrosarcoma cell line(HT1080), were determined using an MTT assay, and the IC50 values of HCPT to the three tumor cell lines were 16. 37, 16. 73 and 19. 24μg/mL, respectively. The growth inhibitory effect of HCPT on a normal cell line, HEK293T(human embryonic kidney cells 293T), was also investigated, the highest inhibitory rate reached 88. 72% at an HCPT concentration of 40μg/mL, in-dicating that the normal cell line HEK293 T is more sensitive to HCPT in comparison to the three tumor cell lines. Using HeLa cell line as a model, Annexin V-FITC apoptosis detection kit was employed to demonstrate the antitumor mechanism of HCPT. Almost all the cells were simultaneously stained by Annexin V-FITC and PI, the cell membrane was green while the nucleus was red, which indicated that HCPT induced the late apoptosis process of HeLa cells. The distribution of HCPT in HeLa cells was observed by fluorescence micros-copy, which provides scientific basis for its application in cell labeling.
