CAJ | 학술논문

采用顶空固相微萃取( HS-SPME)、索氏提取( SE)、超声辅助提取( UAE)、水蒸汽蒸馏法( SD)结合气相-质谱( GC-MS)联用技术分析蛇莓的挥发性组分。实验对HS-SPME各影响因素进行了优化,最终确定采用65μm聚二甲基硅氧烷-二乙烯基苯( PDMS-DVB)萃取头在90℃水浴50 min后插入250℃ GC 进样口解吸5 min,得到最佳提取效果。 HS-SPME, SD, SE和UAE方法分别得到47,32,16和16种挥发性组分,总数为66种,其中47种化合物为首次报道。实验结果表明,HS-SPME与SD得到多为萜类化合物,而SE与UAE得到的化合物中酸类分别占总成分61.44%与69.54%。
채용정공고상미췌취( HS-SPME)、색씨제취( SE)、초성보조제취( UAE)、수증기증류법( SD)결합기상-질보( GC-MS)련용기술분석사매적휘발성조분。실험대HS-SPME각영향인소진행료우화,최종학정채용65μm취이갑기규양완-이을희기분( PDMS-DVB)췌취두재90℃수욕50 min후삽입250℃ GC 진양구해흡5 min,득도최가제취효과。 HS-SPME, SD, SE화UAE방법분별득도47,32,16화16충휘발성조분,총수위66충,기중47충화합물위수차보도。실험결과표명,HS-SPME여SD득도다위첩류화합물,이SE여UAE득도적화합물중산류분별점총성분61.44%여69.54%。
ThevolatilecompositionsofDuchesneaindicawerestudiedbyheadspacesolid-phase microextraction ( HS-SPME ) , soxhlet extraction ( SE ) , ultrasonic assistant extraction ( UAE ) and steam distillation ( SD) coupled with gas chromatography-mass spectrometry ( GC-MS) . The experimental parameters of HS-SPME, including fiber type, extraction temperature, extraction time and desorption time were investigated. 47, 32, 16 and 16 compounds were identified by HS-SPME, SD, SE and UAE extracting methods, respectively. 66 compounds were obtained in total, among which 47 compounds were first reported in Duchesnea indica. The experimental results showed that terpenoids were the most abundant compositions in HS-SPME and SD, but acids accounted for 61. 44% and 69. 54% of the total content obtained by SE and UAE.