CAJ | 학술논문

将鸡蛋清溶液作为“待定模板”制备分子印迹聚合物，得到的聚合物作为色谱固定相，显示出能脱除高丰度蛋白质的能力。采用不同浓度的鸡蛋清进行印迹，可以得到具有不同蛋白脱除性质的聚合物。经过实验室自制的注射器色谱系统处理，蛋清中的高丰度蛋白质(如鸡卵清蛋白、溶菌酶、转铁蛋白)可从相应样品溶液当中去除。随着这些高丰度蛋白质谱峰消失，其它组分的质谱信号变得更加明显。根据文献结果及实验所得质谱数据，判定这些蛋白质分别是卵清白蛋白关联蛋白、转铁蛋白关联蛋白质、卵粘蛋白及黄素蛋白。实验表明，待定模板印迹方法具有脱除高丰度蛋白质，并同时保留、富集低丰度修饰蛋白质的能力。
장계단청용액작위“대정모판”제비분자인적취합물，득도적취합물작위색보고정상，현시출능탈제고봉도단백질적능력。채용불동농도적계단청진행인적，가이득도구유불동단백탈제성질적취합물。경과실험실자제적주사기색보계통처리，단청중적고봉도단백질(여계란청단백、용균매、전철단백)가종상응양품용액당중거제。수착저사고봉도단백질보봉소실，기타조분적질보신호변득경가명현。근거문헌결과급실험소득질보수거，판정저사단백질분별시란청백단백관련단백、전철단백관련단백질、란점단백급황소단백。실험표명，대정모판인적방법구유탈제고봉도단백질，병동시보류、부집저봉도수식단백질적능력。
Eggwhitesolutionswereusedas“pendingtemplates”topreparemolecularimprintedpolymers. The obtained polymers acted as stationary phases, exhibiting abilities to deplete abundant proteins in the egg white. The abilities of protein depletion of the imprinted polymers could be adjusted by using different concentrations of egg white in preparing the polymers. After chromatographically processed in a home-made syringe system, abundant proteins such as ovalbumin, lysozyme, and ovotransferrin were removed from egg white sample solutions. Along with the disappearance of the mass spectrum peaks of these proteins, the signals of other components became more obvious. Meanwhile according to reference data and molecular weights, they were reasonably ascribed to be ovalbumin-related proteins, ovotransferrin-related proteins, ovomucoids or flavoproteins respectively. Conclusively, pending imprinting method has the power to deplete abundant proteins, and to retain and enrich posttranslationally modified species at the same time.